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Buffers:- 2X buffer10 ml 0.5 M imidazol, pH 6.60.21 g LiCl1.25 ml 1 M MgCl21.0 ml 0.1 M EGTA, pH 6.6--> Bring volume up to 50 ml with distilled water.- dilution buffer1 ml 0.5 M imidazol, pH 6.62.5 ml 20 mM diethylenetriaminepentaacetic acid (DTPA)--> Bring volume up to 50 ml with distilled water.Reaction Mixture:solution [stock] vol/tube # samples total vol.2X buffer - 50 μl X _______ = _______DTT 1 M 0.2 μl X......閱讀全文
如果您的實驗需要構建雙熒光素酶報告載體，我們有下列產品供您挑選： 1.我們有雙熒光素酶報告載體產品 產品名稱 貨號 規格 產
一、引言在許多的細胞生命活動中，例如DNA復制、mRNA轉錄與修飾以及病毒的感染等都涉及到DNA與蛋白質之間的相互作用的問題。重組DNA技術的發展，人們已分離到了許多重要的基因。現在的關鍵問題是需要揭示環境因子及發育信號究竟是如何控制基因的轉錄活性。為此需要：a、鑒定分析參與基因表達調控的DNA元件
Bradford protein assayConsiderations for useThe Bradford assay is very fast and uses about the same amount of protein as the Lowry assay. It is fairly
Bovine Ig(Total Immunoglobulin) ELISA KitCatalogue No: EB0001 Size: 48T/96T Reactivity: BovineDetection Range: 1.563-100ug/mlSensitivity: <0.938ug/
Figure 2. Silencer ? siRNA Validation Data Generated Using Applied Biosystems TaqMan? Gene Expression Assays. The indicated Silencer Validated siRNAs
Bradford Protein Concentration Assayversion 01/07/2001Abbreviations:mcg = microgramsmcL = microlitersBSA = bovine serum albuminO.D. = optical densityd
一、引言 在許多的細胞生命活動中，例如DNA復制、mRNA轉錄與修飾以及病毒的感染等都涉及到DNA與蛋白質之間的相互作用的問題。 重組DNA技術的發展，人們已分離到了許多重要的基因。現在的關鍵問題是需要揭示環境因子及發育信號究竟是如何控制基因的轉錄活性。為此需要： a、鑒定分析
實驗概要The experiment provides a simple, fluorescence-based in vitro assay for detecting the GST activity using a fluorescence plate reader.
賽默飛世爾科技公司近日宣布，適用于癌癥研究的靶向檢測產品線又增添四款新產品，包括Oncomine Immune Response Research Assay，它能夠拷問腫瘤微環境，并鑒定免疫治療臨床試驗中的預測性標志物。這一系列產品適用于Ion Torrent NGS平臺，與FFPE樣品兼容。
2.2 Interaction mating - large scaleWith a few modifications, the procedure described above can be used to test for interactions between a single prey
AbstractA general method for the assay of deubiquitinating enzymes was described in detail using 125I-labeled ubiquitin-fused αNH-MHISPPEPESEEEEE
Genome-wide Gene Expression Analysis (Richard Young Research Group,Whitehead Institute for Biomedical Research)A genoe-wide gene expression analy
核心提示：關于牛奶中IGF-1對人體的影響，美國食品藥品局（FDA）早已經做出了結論。2007年5月，在FDA最新公布、經美國國家審計署審計的報告中寫道：“IGF-1與癌癥無關，牛奶中IGF-1濃度的變化不會增加人體罹患癌癥的幾率”。
Protein AssaysBelow is a list of assays for the determination of protein concentration in a solution. This list includes the sensitivity range, volume
實驗概要a protocol for Leaf GUS Assay This protocol is for small samples (usually single leaf from 21DAI plants), scale up for larger samplesAs
cAMP/cGMP（環磷酸腺苷/環磷酸鳥苷）是一種環狀核苷酸，以微量存在于動植物細胞和微生物中。有人稱其為細胞內的第二信使，而稱激素為“第一信使”。cAMP（或cGMP）由腺苷酸（或鳥苷酸）環化酶產生，而被磷酸二酯酶降解，因此激活腺苷酸（或鳥苷酸）環化酶或者抑制磷酸二酯酶可以提高細胞內c
Carbohydrate AssaysREFERENCE: Wright and Rebers, Anal. Biochem. 49: 307-319, 1972.OBJECTIVE: To determine the relative amounts ofLPS carbohy
IntroductionMature T cells recognize and respond to the antigen/MHC complex through their antigen-specific receptors (TCR). The most immediate consequ
In Vitro T Cell ActivationIntroductionMature T cells recognize and respond to the antigen/MHC complex through their antigen-specific receptors (TCR).
overviewThe Under-Agarose assay is a useful method for observing the response of a cell population to one or more chemoattractant sources. The behavio
With the aid of spectroscopy, the quantitative analysis of nucleic acids and proteins has established itself as a routine method in many laboratories.
Our experience indicates that it may not be sufficient to change the medium containing Rottlerin and to wash the cells before adding MTT to avoid a po
Because an immediate sign of mitochondrial uncoupling is the drop in cell phosphorylation potential, we evaluated the Rottlerin uncoupling effect by d
With the aid of spectroscopy, the quantitative analysis of nucleic acids and proteins has established itself as a routine method in many laboratories.
With the aid of spectroscopy, the quantitative analysis of nucleic acids and proteins has established itself as a routine method in many laboratories.
簡介體外監測細胞的增殖對于基礎研究和應用研究都極有價值并有潛力應用在臨床領域。 3H-thymidine 或 5-bromo-deoxyuridine (BrdU, 胸苷的類似物 ) 摻入法是確定培養細胞的增值率的傳統方法，這主要是因為幾乎沒有更方便的替代方法。因而人們投入了大量的精力來開發一種快速
2018年7月19日，上海——科學服務領域的世界領導者賽默飛世爾科技（以下簡稱：賽默飛）宣布推出基于高通量測序技術（NGS）的Oncomine TCR Beta-SR Assay（SR = Short Reads）試劑。該產品可用于全面評估免疫系統的多樣性，從而評價人體對感染、疫苗、細胞免疫治療
一、實驗試劑 GUS Buffer (500 ml) 2.0478 g Na2HPO4 1.2688 g NaH2PO4 (=50 mM NaPi pH7.0) 10 ml 0.5 M EDTA (=10 mM) 0.5 g &n
IntroductionMature T cells recognize and respond to the antigen/MHC complex through their antigen-specific receptors (TCR). The most immediate consequ
Note: Samples to be used within 5 days can be stored at 4°C, besides that, samples must be stored at -20°C (assay ≤1 month) or -80°C(assay≤2 month