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IntroductionMature T cells recognize and respond to the antigen/MHC complex through their antigen-specific receptors (TCR). The most immediate consequence of TCR activation is the initiation of signaling pathways including induction of specific protein tyrosine kinases (PTKs), breakdown of phosphatidylinositol 4,5-biophosphate (PIP2), activation of protein kinase C (PKC) and elevation of intracellular calcium ion con......閱讀全文
For a T cell to be activated by a specific antigen, the T cell receptor must recognize complexes of MHCI with the antigen on the surface of an antigen
Interaction of T cell receptor with specific antigen in the context of MHC II activates a signal transduction pathway that leads to T cell activation.
In Vitro T Cell ActivationIntroductionMature T cells recognize and respond to the antigen/MHC complex through their antigen-specific receptors (TCR).
IntroductionMature T cells recognize and respond to the antigen/MHC complex through their antigen-specific receptors (TCR). The most immediate consequ
實驗概要The CellTrace? Violet Cell Proliferation Kit provides a versatile and well-retained cell tracing reagent in a convenient and easy-to-u
ECM Cell Attachment Assay (LTI)Cell Adherence Inhibition Assay (LTI)General protocol--Either monoclonal antibody or RGD peptide is added alo
實驗概要A modification of the basic immunofluorescence staining and flow cytometric analysis protocol can be used for the simultaneous analysi
實驗概要Central neurons lose the ability for axonal regrowth during development and typically do not regenerate their axons following axotomy once the
來自中國科技大學的研究人員證實，TIP60介導Aurora B乙酰化確保了染色體正確分離。這一研究發現發布在2月1日的自然化學生物學（Nature Chemical Biology）雜志上。 中國科技大學的姚雪彪（Xuebiao Yao）教授和Xing Liu是這篇論文的共同通訊作者。姚教授
PurposeThe main purpose of the flow chamber assay is to visualize and measure interactions between flowing cells expressing a given adhesion molecule
IntroductionEnzyme-linked immunosorbent spot (ELISPOT) assays were originally developed to enumerate B cells secreting antigen-specific antibodies, bu
Cytokine ELISPOT ProtocolDescriptioneBioscience ELISPOT Ready-SET-Go! reagent sets contain the necessary reagents for performing enzyme linked immunos
Vascular endothelial growth factor (VEGF) plays a key role in physiological blood vessel formation and pathological angiogenesis such as tumor growth
IntroductionIn our lab we use the term 'cell-free system' when we talk about the examination of apoptotic activity in cytoplasmic extrac
CD4+ helper T cells differentiate into distinct subtypes, Th1 and Th2 cells. Th2 cells are involved in the response to extracellular helminthe parasit
實驗概要This protocol is intended for activation and expansion of human Treg cells isolated with the Dynal? CD4 CD25 Treg Kit (Cat. no. 113.23D). The exp
IntroductionA modification of the basic immunofluorescent staining and flow cytometric analysis protocol can be used for the simultaneous an
Proline-,glutamic acid-,leucine-rich protein 1 (Pelp1 also known as:modulator of nongenomic activity of estrogen or MNAR) is a recently discovered tra
Figure 5: Regulation of CDH5 by TFZFs in several human cancer cell lines.Blue, cells infected with a pMX construct containing the DNA bindin
英語謂語動詞時態共有16種，在英文科技論文中用得較為頻繁的主要有三種：即一般現在時、一般過去時和將來時。正確地使用動詞時態是科研寫作的基本功，我們在撰寫英文論文時，如不能正確選用時態，常常會改變文章所要表達的意思，從而影響評審專家與讀者的理解。 一篇典型的科技論文有一個基本的框架結構：Abstrac
Many cell-surface receptors induce production of second messengers like PIP3, phosphatidylinositol 3,4,5-trisphosphate, that convey signals to the cyt
Nerve growth factor (NGF) is a neurotrophic factor that stimulates neuronal survival and growth through TrkA, a member of the trk family of tyrosine k
Table 2: Human Cytokines: Intracellular Staining Quick GuideHuman Cytokines: Intracellular Staining Quick GuideHuman CytokineCell SourceActivationIncu
一、熒光素酶報告基因的檢測原理熒光素酶（Luciferase）是生物體內催化熒光素(luciferin)或脂肪醛(firefly aldehyde)氧化發光的一類酶的總稱，來自于自然界能夠發光的生物。自然界存在的熒光素酶來自螢火蟲、發光細菌、發光海星、發光節蟲、發光魚、發光甲蟲等。細菌熒光素酶對熱敏
The complement cascade of proteolytic factors involved in cellular lysis can be initiated by several different factors, including antibody-dependent a
2.2 Interaction mating - large scaleWith a few modifications, the procedure described above can be used to test for interactions between a single prey
IL-22 is an inflammatory cytokine related to IL-10 that is produced by T cells and that induces a response in cells through a heterodimeric cell surfa
Nonyl Acridine OrangeNonyl acridine orange (A1372) is well retained in the mitochondria of live HeLa cells for up to 10 days, making it a useful probe
Studies with homozygous knockout mice have suggested that stem cell factor (SCF or KITLG), c-Kit, microphthalmia (Mitf), and B-cell CLL/lymphoma 2 (Bc
Activation of the MAPK kinase pathway has been identified as a mechanism that integrins use to regulate gene expression leading to cell shape changes