MethodsfortheMeasurementofaBacterialEnzymeActivityinCellLysates
AbstractThe kinetic characteristics and regulation of aspartate carbamoyltransferase activity were studied in lysates and cell extracts of Helicobacter pylori by three diffirent methods. Nuclear magnetic resonance spectroscopy, radioactive tracer analysis, and spectrophotometry were employed in conjunction to identify the properties of the enzyme activity and to validate the results obtained with each assay......閱讀全文
Methods for the Measurement of a Bacterial Enzyme Activity in Cell Lysates
Abstract The kinetic characteristics and regulation of aspartate carbamoyltransferase activity were studied in lysates and cell extracts of?He
Methods for the Measurement of a Bacterial Enzyme Activity in Cell Lysates3
Different enzyme assays for ACTase study in H. pylori ACTase properties were studied?in situ?in cell-free extracts to obtain information on enzyme f
Methods for the Measurement of a Bacterial Enzyme Activity in Cell Lysates4
The use of a micro-titre based colorimetric assay provided a third method for the study of ACTase activity, and the most useful for large scale measu
Methods for the Measurement of a Bacterial Enzyme Activity in Cell Lysates2
Measurement of ACTase activityNuclear magnetic resonance spect roscopy (NMR)The unique potential of NMR spectroscopy for monitoring simultaneously the
Hydrolytic Activity of Bacterial Supernatants for Fungal Suppression
As the fungal growth suppression by biocontrol agents (BCA) in solid media using dual plate assay has some issues regarding nutrient limitation. A pr
Histochemical staining of sea urchin embryos for (AP) enzyme activity
Histochemical staining of sea urchin embryos for alkaline phosphatase (AP) enzyme activity1. Obtain embryo samples, tube of?AP substrate buffer?and tu
Bacterial cell culture
Materials Glass culture tubes with metal caps and labels Growth medium, from media room or customized Glass pipette tubes Parafilm Equ
Staining Methods for cell
death Z. Xia 10/2/95 The simplest way: trypan blue.? Dead cells stain blue Non-fixed cells: FDA(fluorescein diacetate)-green, alive cells;?
Measurement of Cell Adhesion Under Static Conditions
Many different molecules have been described to promote cell adhesion including several cell surface carbohydrate-binding proteins. Measuring cell ad
Staining Methods for cell death
The simplest way: trypan blue.? Dead cells stain blue Non-fixed cells: FDA(fluorescein diacetate)-green, alive cells;? P.I. (propidium iodide)-r