Method: Maintaining Lymphoblastoid Cell LinesJune 10, 1990Rosalie VeilePurpose:To grow lymphoblastoid cells for permanent storage and for DNA extraction.Safety Considerations:All cultured animal and human cells have the potential for carrying viruses, latent viral genomes, and other infectious agents. Cell cultures should be handled very carefully by trained persons under laboratory conditions which afford adequate b......閱讀全文
常規操作(主要內容如下)· Aseptic Technique· Culture Ves
· Lymphocyte Transformation (Donis-Keller lab)Lymphocytes are transformed to establish cell