Invitrocultureofembryoniclungs
In vitro culture of embryonic lungsfrom Hogan LabIsolation of Lung Bud EndodermWhat you need:E11-12 mouse embryosDMEM with 5% fetal bovine serumpetri dishes for dissections and washesTyrode-Ringer's solution, pH 7.6-7.7 (recipe below)pancreatin-trypsin solution (recipe below)1. Dissect E11-12 lungs in DMEM and 5% serum on ice. Young lungs (E11 to 11.5) are best.2. Wash the lungs 3 x 5 minutes on ice in Tyrode-Rin......閱讀全文
In vitro culture of embryonic lungs
In vitro culture of embryonic lungs from Hogan Lab Isolation of Lung Bud Endoderm What you need: E11-12 mouse embryos DMEM with 5% fetal bov
Embryonic limb bud culture in media
Early in embryonic development, the region of the chick embryo which is determined to form a limb first differentiates from the rest of the embryo1. T
Culture of Human Embryonic Stem Cells (hESC)
All cell lines are initially grown according to the supplier's protocols but we are adapting them to one simple protocol outlined below: 6-well pl
Human Embryonic Stem (ES) Cell Protocols——General notes on ES cell culture
hES media has a two week shelf life. hES cells should be cultured in 4, 6, 24, 48, 96 well plates. Growing cells in flasks is not recommended be
Human Embryonic Stem (ES) Cell Protocols——Embryonic Bodies
Let human ES cells grow until the colonies are large and the cells are pretty piled up - about the time when you would normally split or even a day pa
In Vitro Fertilization
When we first started using X. tropicalis,?in vitro?fertilization had an extremely poor efficiency. However, with the careful selection of a mature m
Stem cell characteristics of amniotic epithelial (AE) cells
Isolation of AE Cells1.?Human placentae were obtained with the approval of the institutional review board, after uncomplicated elective caesarean
胚胎干細胞培養
Media and Solution required for ES Cell Culture?(Bowtell Lab)???Routine Culturing of ES Cells?(Bowtell Lab)??Routine Splitting and freezing of cells?(
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic-1
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In VitroRoxanne Holmes?and?Juan Carlos Zú?iga-Pflücker1Sunn
In vitro Sphingomyelinase Assay
Reagents: Lysis buffer 25 mM Tris-HCl, pH 7.4 5 mM EDTA 1 mM ATP 20 μg/ml CLAP 1 mM PMSF Buffer A 10 mM MgCl2 0.2 M Tris-HCl, pH 7.4 0.2 % Triton X