Method:RemovalofYeastContaminationfromLymphoblastCultures
Purpose:This method is advantageous for saving the occasional cultures that become contaminated. Yeast contaminated cultures will appear cloudy when slightly shaken and lymphocytes will not cluster together as much as normal. If cultures are suspect, a drop of culture can be streaked on a YPD media plate to check for growth of yeast colonies, or a 5 ml sample can be taken to Barnes Diagnostic Center for identificatio......閱讀全文
Method: Removal of Yeast Contamination from Lymphoblast Cultures
Purpose:This method is advantageous for saving the occasional cultures that become contaminated. Yeast contaminated cultures will appear cloudy when s
細胞培養常規操作
常規操作(主要內容如下)·?????????Aseptic Technique·?????????Culture Vessels·?????????Cell Counting·?????????Primary Culture·?????????Maintenance of Cell Line?·??
Direct PCR from Whole Yeast Cells: Zymolyase Method
Direct PCR from Whole Yeast Cells: Zymolyase MethodContributor: Namjin ChungDate: June 18, 19961. An average-size yeast colony (0.5-2mm) or a cell pel
Preserving yeast cultures
Short term storage Yeast cultures are stable for 1-2 weeks when refrigerated. Petri dishes should be sealed or in plastic bags. Medium term stora
Sodium Azide removal from antibody solutions
實驗概要Sodium azide is a preservative used for inhibiting the growth of contaminants such as bacterial or fungus in antibody solutions. However, its
Plasmid isolation from yeast
Pick colonies into 0.5ml of SD-Leu (or other appropriate SD medium) Vortex for 1min Leave to grow O/N for 18-24h at 30°C, 230-250rpm (best in 5ml b
Decontamination of cells from the yeast
I???? Destroy yeast 1.???? Aspirate medium and wash cell in PBS. 2.???? Incubate cells at 37oC for 5 min in non-diluted antibiotic-antimycotic. 3.
A safe method of extracting DNA from Coccidioides immitis
Human-pathogenic fungi such as Coccidioides immitis and Histoplasma capsulatum must be handled in Biosafety level 3 containment facilities which make
Quick and Easy Isolation of Genomic DNA from Yeast
Procedure Transfer 1.5 ml of liquid culture of yeast grown for 20 - 24 h at 30°C in YPD (1% yeast extract, 2% peptone, 2% dextrose) into a microce
Method: Maintaining Lymphoblastoid Cell Lines
Method: Maintaining Lymphoblastoid Cell Lines June 10, 1990 Rosalie Veile Purpose: To grow lymphoblastoid cells for permanent stora