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Serum Free Media for human ES cells on MEFs: can last for 7-10 daysFinal ConcentrationAmount for 250ml Stock solution80% DMEM-F12200ml20% KO Serum Replacer50ml1% Non-essential Amino Acids2.5ml1mM L-glutamine (see recipe)2.5ml0.1mM b-mercaptoethanolpart of L-glutamine4ng/ml bFGF (see recipe)0.5mlL-glutamine stock solution: Prepare on a per-use basis0.146g L-glutamine10ml Ca/Mg free PBS7 ul b-mercaptoethanolb......閱讀全文
Noninvasive Human Nuclear Transfer with Embryonic Stem CellsSohyun L. McElroy1 and Renee A. Reijo PeraCenter for Human Embryonic Stem Cell R
結論· 我們在C1TM單細胞自動制備系統開發了一種簡潔的實驗方案，能以最少的手工操作，在不到24小時內，平行處理高達96個單細胞，對其miRNA表達譜進行分析。· C1 miRNA STA實驗方案使用了Life Technologies為miRNA優化過的試劑。特別的，Meg
IntroductionHuman embryonic stem (hES) cells are pluripotent stem cells derived from pre-implantation embryos that can be maintained and expanded in a
Introduction MicroRNA (miRNAs) are short (18–24 nucleotides), non-coding RNAs that regulate gene expression by both disrupting mess
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In VitroRoxanne Holmes and Juan Carlos Zú?iga-Pfl
Lentivirus Transduction of Hematopoietic CellsMing-Jie Li and John J. RossiDivision of Molecular Biology, Beckman Research Institute of the City of Ho
If culturing in the absence of a feeder cell layer is desired, human embryonic stem (hES) cells can be maintained using Mouse or Human-Conditioned Med
生物分子發表的代表性文獻 QTRAP：同時具有三重四極桿和線性離子阱性能的獨一無二的LC/MS/MS系統 QTRAP系統最早在ASMS 2002上，作為第一臺商用的線性離子阱發布，是世界上唯一的線性離子阱和三重四極桿的復合
Metabolic biotinylation of mammalian cell receptors for imagingBakhos A. Tannous , btannous@hms.harvard.edu, Massachusetts General Hospital and Harvar