ApoptosisTUNELassay(ParaffinSections)
Protocol for Paraffin Sections:Dewax paraffin sections:Incubate slides, 55°C, 30 min.Xylenes, 2 times, 2 min. each100% EtOH, 2 times, 2 min. each95% EtOH, 2 times, 2 min. each80% EtOH, 2 min.75% EtOH, 2 min.50% EtOH, 2 min.dH2O rinse.Incubate slides in a 1μg/ml Proteinase K/10mM Tris solution, 15 min., RT. (7.5μl of 20μg/μl PK in 150 ml 10mM Tris, pH 7.4-8.0).All slides: 1x PBS rinse, 2 times (+ 10 min for those non-......閱讀全文
Apoptosis TUNEL assay (Paraffin Sections)
Protocol for Paraffin Sections: Dewax paraffin sections: Incubate slides, 55°C, 30 min. Xylenes, 2 times, 2 min. each 100% EtOH, 2 times, 2 min.
Apoptosis TUNEL Assay (frozen sections)
Protocol for Frozen Sections:Warm 150ml 4% Paraformaldehyde/1x PBS to RT. Fix slides in it, 20 min., RT.1x PBS rinse, 2 times.1x PBS, 30 min., RT. Beg
TUNEL assay
PROTOCOL:?Deparaffinize and rehydrate slides:3 x 3′ Xylene3 x 2′ 100% ethanol1 x 2′ 95%, 80%, 70% ethanol (each)1 x 5′ 1x PBS?Microwave antigen retrie
In Situ Cell Death (Apoptosis) Detection by TUNEL labeling
Protocol for Frozen Sections:Warm 150ml 4% Paraformaldehyde/1x PBS to RT. Fix slides in it, 20 min., RT.1x PBS rinse, 2 times.1x PBS, 30 min., RT. Beg
Guide to Cell Proliferation and Apoptosis Methods
Chapter 1: Cell Death - Apoptosis and Necrosis 1.1 Introduction 2 1.1.1 Terminology of cell death 2 1.1.2 Differences between necros
Apoptosis: A Laboratory Manual of Experimental Methods Andrea Cossarizza
THE CELL?1.?Morphological aspects of apoptosis?Walter Malorni, Stefano Fais & Carla Fiorentini?2.?Cell cycle?Miriam Capri & Daniela BarbieriTHE NUCLEU
凋亡細胞核DNA片段檢測方法進展（二）
2.3 凋亡細胞的TUNEL和ISNT鑒定的流式細胞儀分析[16]對于培養的細胞，可以將TUNEL或ISNT鑒定同流式細胞儀結合起來分析其發生凋亡的情況。待檢細胞與含有TdT或DNA聚合酶I或Klenow片段及生物素標記的dUTP反應液共孵育一段時間后，加入熒光素（常用FITC）標記的鏈霉抗生物
Apoptosis: Miniassay
1) Aliquot 5 X 106 total cells in 5-8 ml 2% media. 2) Add 30 μl 3H-thymidine. 3) Incubate for approximately 16 hours under normal growth conditions
Apoptosis Induction
IntroductionWhen studying induction of apoptosis via a cell surface molecule, it is important to first ascertain surface expression of the molecule of
Detection of apoptotic process in situ using immunocytochemical
1. INTRODUCTION? ? Apoptosis was observed from invertebrates to lower and higher verterbrates, and intervenes both in physiological and in pat