CountingEScellChromosomes
(original reference: "tissue culture made easy" by Christian LaMantia from the Magnuson lab)1) Plate cells onto gelatinized plates (35 or 60 mm) without feeders and culture 24 hours. A 1:4 split is best for most lines.2) Culture for four hours in the presence of colcemid (3.125 μl of 10 μg/ml colcemid (Gibco # 15212-012) per ml of medium). 2ml per 35 mm and 4ml per 60 mm.3) Remove media and place it in a 15......閱讀全文
Counting ES cell Chromosomes
(original reference: "tissue culture made easy" by Christian LaMantia from the Magnuson lab) 1) Plate cells onto gelatinized plates (35 or 60 mm) wi
Cell counting/plating
OverviewCount the number of viable cells in a culture via dilution plating. The following assumes E. coli cells but it should apply to any type of cel
Cell counting with an hemacytometer.
Accuracy of manual counts with an hemacytometer depend on:accurate mixing of the sample (no bubbles!)number of chambers countednumber of cells counted
Cell Counting/ Live-Dead Discrimination
This is a microscopy based application for definitive discrimination of live and dead cells. Trypan Blue exclusion notoriously over estimates the num
Human Embryonic Stem (ES) Cell Protocols——General notes on ES cell culture
hES media has a two week shelf life. hES cells should be cultured in 4, 6, 24, 48, 96 well plates. Growing cells in flasks is not recommended be
ES Cell Culture and Manipulation
MediaHigh glucose DMEM (-pyruvate, -glutamine)20% Heat-inactivated Fetal calf serum (can vary by cell type, be sure!!)1X l-glutamine1X Penicillin/stre
ES and TS cell freezing/thawing
Needed: ES cell freezing medium (2x) 2x ES cell freezing medium should be made up fresh each time it is to be used, and should comprise freshly pre
ES and TS cell freezing/thawing
實驗概要ES and TS cell freezing/thawing.主要試劑ES cell freezing medium (2x)? ? ? ? 2x ES cell freezing medium should be made up fresh each time it is to be
Human Embryonic Stem (ES) Cell Protocols——Freezing Human ES Cells
? Collagenase cells for approximately 7 minutes at 37 °C (until edges of colonies are curling up). With a 5 ml pipet, gently pipet and scrape colon
Human Embryonic Stem (ES) Cell Protocols——Thawing Human ES cells
Remove Human ES cells from liquid nitrogen storage tank. Fill out a freeze/thaw form.Thaw cryovial by gently swirling in waterbath until only a small