WIKI資訊
We use feeder-independent ES cell lines derived from the 129/Ola strain of mice (Nichols et al., Development 110, p.1341, 1990). These cells are easy to maintain and significantly reduce the amount of tissue culture required. Parental cell lines (CGR8 and E14Tg2A) were established from delayed blastocysts on gelatinized tissue culture dishes in ES cell medium containing leukocyte inhibitory factor (LIF) (Nichols et a......閱讀全文
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In VitroRoxanne Holmes and Juan Carlos Zú?iga-Pfl
Lentivirus Transduction of Hematopoietic CellsMing-Jie Li and John J. RossiDivision of Molecular Biology, Beckman Research Institute of the City of Ho