Media UsedTo prepare 100 ml mediumDMEM80 mlFCS15 mlNon-essential amino acids (100x)1 mlPen/strep (5,000 1U/ml, 5000 ug/ml)1 mlL-Glutamine 200 mM1 mlNucleosides stock (100x)1 mlBME 0.1M0.2 mlDulbecco's Modification of Eagles Medium (DMEM)1 x without L-glutamine with 4.5 g/L Glucose. Store at 4Cytosystems 500 ml Cat. No. 11.016.0500VNon essential amino acids 100xCytosystems 100 ml Cat No. 21-145-0100V. Store at 4......閱讀全文
Media and Solution required for ES Cell Culture (Bowtell Lab) Routine Culturing of ES Cells (Bowtell Lab) Routin
MEDIA AND SOLUTIONS REQUIRED FOR ROUTINE ES CELL CULTURERoutine Culturing of ES CellsISOLATION OF PRIMARY MOUSE EMBRYO FIBROBLASTSMITOMYCIN C TREATMEN