線粒體熒光探針大全:TMRM,Mitotracker,JC1(6)
Pyruvate DehydrogenaseMolecular Probes has available a series of antibodies against the human pyruvate dehydrogenase (PDH) complex (Table 12.6), a large, multienzyme assembly residing in the mitochondrial matrix and consisting of three catalytic activities: pyruvate dehydrogenase, dihydrolipoyl transacetylase and dihydrolipoyl dehydrogenase 193 (diaphorase). The PDH complex is responsible for the oxidative ......閱讀全文
線粒體熒光探針大全:TMRM,Mitotracker,JC-1(6)
Pyruvate DehydrogenaseMolecular Probes has available a series of antibodies against the human pyruvate dehydrogenase (PDH) complex (Table 12.6), a lar
線粒體熒光探針大全:TMRM,Mitotracker,JC-1(3)
Mitochondrion-Selective Rhodamines and RosaminesRhodamine 123Rhodamine 123 (R302; FluoroPure Grade, R22420; Figure 12.22) is a cell-permeant, cationic
線粒體熒光探針大全:TMRM,Mitotracker,JC-1(1)
線粒體熒光探針信息大全 (Probes for Mitochondria)包括各種常用探針,如JC-1,JC-9,TMRM,TMRE等Mitochondria are found in eukaryotic cells, where they make up as much as 10% of th
線粒體熒光探針大全:TMRM,Mitotracker,JC-1(4)
Nonyl Acridine OrangeNonyl acridine orange (A1372) is well retained in the mitochondria of live HeLa cells for up to 10 days, making it a useful probe
線粒體熒光探針大全:TMRM,Mitotracker,JC-1(5)
Monoclonal Antibodies Specific for Proteins in the Oxidative Phosphorylation SystemOxidative phosphorylation (OxPhos) activity occurs in the mitochond
線粒體熒光探針大全:TMRM,Mitotracker,JC-1(2)
MitoTracker Green FM ProbeMitochondria in cells stained with nanomolar concentrations of our patented MitoTracker Green FM dye (M7514) exhibit bright
線粒體膜電位熒光探針-Cell Meter 線粒體膜電位(MMP)
人體的ATP有95%為線粒體所提供,合成的ATP通過線粒體內膜ADP/ATP載體與細胞質中的ADP交換進入細胞質,參與細胞的各種需能過程,因此線粒體與細胞維持正常功能密切相關。線粒體在呼吸氧化過程中,將所產生的能量以電化學勢能儲存于線粒體內膜,在內膜兩側造成質子及其他離子濃度的不對稱分布而形成線粒體
細胞凋亡檢測實驗——線粒體膜勢能的檢測
實驗方法原理線粒體在細胞凋亡的過程中起著樞紐作用,多種細胞凋亡刺激因子均可誘導不同的細胞發生凋亡,而線粒體跨膜電位的下降,被認為是細胞凋亡級聯反應過程中最早發生的事件,它發生在細胞核凋亡特征(染色質濃縮、DNA 斷裂)出現之前,一旦線粒體DYmt 崩潰,則細胞凋亡不可逆轉。?線粒體跨膜電位的存在,使
LSCM細胞亞微結構
細胞亞微結構(細胞器探針)一般的光學顯微鏡由于分辨率有限,在觀察細胞器結構時受到一定的限制,而共聚焦激光掃描顯微鏡可獲得較一般普通光學顯微鏡分辨率高的細胞內線粒體、高爾基復合體、內質網、溶酶體等細胞器圖像,同時還可動態觀察活細胞狀態下細胞器的形態學變化情況,此外還可通過光學切片即斷層掃描技術進行三維
細胞凋亡的檢測方法綜述-5
4、檢測細胞膜成分變化的Annexin V 聯合PI法原理:磷脂酰絲氨酸(Phosphatidylserine, PS)正常位于細胞膜的內側,但在細胞凋亡的早期,PS可從細胞膜的內側翻轉到細胞膜的表面,暴露在細胞外環境中(圖3)。Annexin-V是一種分子量為35~36KD的Ca2+依賴性磷脂