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Cell CultureMaterials1. Falcon Primaria culture dishes.2. Culture medium: DME (or F12K) with glutamine, supplemented with 7% heat-deactivated horse serum (56oC for 30 min), 7% fetal calf serum, 50 units penicillin, 50 ug/ml streptomycin, 37oC.3. ECL matrix (E-C-L cell attachment matrix, Upstate Biotech, 08-110). Stored as 250 ul aliquots in 15 ml conical tubes at -80oC. Keep it cold all the time otherwise it will for......閱讀全文
Top of pageProcedureOverviewSteps 1 - 8 Preparation of rat brain cytosolSteps 9 - 10 Culture of PC12 cellsSteps 11 - 30 Preparation of postnuclea
Full size image (70 KB)In vitro incubation of a reaction mix that contains labeled endosomes, cytosol and an ATP-regenerating system at phys