WIKI資訊
3’ Adaptor Ligation and PurificationHeat shock the RNA by putting at 90°C for 30 seconds. Snap cool on ice.Set up the 3’Adaptor ligation reaction in a 1.5 ml siliconized tube: Purified 5’ ligation product from step 2.15 6.4μl3’ RNA adaptor (10μM) ......閱讀全文
My Experience Purifying RNA from E. coliRegarding RNA extraction, there is a horrible tendency of people to use kits for RNA extraction with bacteria.
實驗概要This method is use to extract short RNAs from plant tissue. Some of the variables (e.g. centrifugation speeds×, precipitation
核仁小RNA(snoRNAs)是一類中等長度的非編碼小RNA,它們的長度在60-300nt不等，能與核仁核糖核蛋白結合形成snoRNPs 復合物[1]。在脊椎動物中編碼核仁小RNA的基因主要存在于蛋白編碼基因或非蛋白編碼基因的內含子區域，并且經過進一步的轉錄后加工處理形成成熟的核仁小RNA[2]
snoRNAs在癌癥中的作用snoRNAs參與的癌癥的分子病理學研究snoRNAs在癌癥中的作用起始于一項研究：在腦膜瘤中，與正常腦組織相比snoRNAs的表達大幅下調[20]。最近研究發現，在非小細胞肺癌中多種snoRNAs呈現出不同的表達狀態[21]。其它的研究證明snoRNAs U50
實驗概要 This protocol utilizes the powerful guanidine isothiocyanate–phenol:chloroform extraction method which allows the
實驗概要The SuperScript? III One-Step RT-PCR System with Platinum? Taq High Fidelity is designed for sensitive, high-fidelity end-p
實驗概要The following basic protocol serves as a general guideline and a starting point for any PCR amplification. Optimal reaction conditions
Purpose:To isolate intact, high molecular weight DNA from yeast cells for subcloning and rare cutting restriction enzyme analysis. One can expect a yi
Phytohemaglutinin (PHA)Available as kidney bean lectin. It is typically used as a stock solution of 10-20 g/ml in balanced salt solution. For tissue c
Manipulating and analyzing DNA are fundamentals in the field of molecular biology. Indeed, separating complex mixtures of DNA into different sized fra
Direct labeling of total RNA with Cy3 and Cy5:A. MATERIALSRNeasy? Mini Kit (Qiagen; Cat # 74106) SuperScript II RT (200U/μL) (Life Technologies;
INTRODUCTIONThis protocol describes the histological preparation of embryonic and adult zebrafish eyes. The methods described here can be easily adapt
Preparation of nucleic acid probesIn standard nucleic acid hybridization assays the probe is labeled in some way. Nucleic acid probes may be made as s
Cloning PCR ProductsT-A Cloning Strategy: Taq and other polymerases seem to have a terminal transferase activity which results in the n
Cloning PCR ProductsT-A Cloning Strategy: Taq and other polymerases seem to have a terminal transferase activity which results in the non-te
Joseph SambrookPeter Maccallum Cancer Institute and The University of Melbourne, AustraliaDavid W. RussellUniversity of Texas Southwestern Medical Cen
實驗概要 Extension of overlapping gene segments by PCR is a simple, versatile technique for site-directed mutagenesis and g
一個經典的蛋白芯片操作流程：Experimental Procedures for Protein Microarrays--------------------------------------------------------------------------------Chemicall
Introduction The most important aspect of our cloning vectors is that t
OUTLINEWestern blot is a wide used technique to identify a target protein/s for the certain antibody.PROTOCOLPrepare platelets.Lyse washed platelets (
Methylated CpG island Amplification Protocol written by Minoru Toyota2. Materials2.1. MCARestriction enzymes SmaI, XmaIT4 DNA ligaseTaq DNA polym
Quantitative PCRJoseph SambrookPeter Maccallum Cancer Institute and The University of Melbourne, AustraliaDavid W. RussellUniversity of Texas Southwes
EuthanasiaEuthanasia and mouse necropsies require prior IACUC approval. The mode of euthanasia should be chosen which minimizes pain or distress to th
BASIC THEORY AND USE OF GC-MSbyDr. Eugenia SobolevaContent1. Introduction.2. GC-MS syste
Different enzyme assays for ACTase study in H. pyloriACTase properties were studied in situ in cell-free extracts to obtain information on e
DescriptionSingle primer PCR allows amplification from known to unknown regions in chromosomes, phage, plasmids, large PCR products and other sources
Isolation of BAC DNA from Large-scale CulturesJoseph SambrookPeter Maccallum Cancer Institute and The University of Melbourne, AustraliaDavid W. Russe
Magnesium chloride (MgCl MW 95.23)1 mMDissolve 95.2 mg of magnesium chloride per final volume of 1 liter.4 mMDissolve 0.381 grams of magnesium ch
Sample Protopcol for Two-Step Carbodiimide Coupling of Protein to MagPlex? Magnetic Carbo
(This procedure can also be used for virtually any material that must be pelleted prior to fixation and thin sectioning)Primary fix:2% glutaraldehyde