CloningofsmallRNAswith5’phosphateand3’OHends2
3’ Adaptor Ligation and PurificationHeat shock the RNA by putting at 90°C for 30 seconds. Snap cool on ice.Set up the 3’Adaptor ligation reaction in a 1.5 ml siliconized tube: Purified 5’ ligation product from step 2.15 6.4μl3’ RNA adaptor (10μM) ......閱讀全文
Cloning of small RNAs with 5’ phosphate and 3’ OH ends-2
3’ Adaptor Ligation and Purification Heat shock the RNA by putting at 90°C for 30 seconds. Snap cool on ice. Set up the 3’Adaptor ligation reac
Cloning of small RNAs with 5’ phosphate and 3’ OH ends-3
Load your precipitated PCR samples into 2 consecutive lanes so as not to overload the lanes. For each different sample, I would run a separate ladder
Cloning of small RNAs with 5’ phosphate and 3’ OH ends0-1
IntroductionThe following protocol describes a procedure for the purification and cloning of miRNAs and other small RNAs in the? 20-30 nucleotide size
RNAi-制備小分子干擾RNA（small interfering RNAs，siRNAs)的方法-1
最適用于：快速而經濟地研究某個基因功能缺失的表型不適用于：長時間的研究項目，或者是需要一個特定的siRNA進行研究，特別是基因治療體內表達前面的3種方法主要都是體外制備siRNAs，并且需要專門的RNA轉染試劑將siRNAs轉到細胞內。而采用siRNA表達載體和基于PCR的表達框架則屬于：從轉染到細
RNAi-制備小分子干擾RNA（small interfering RNAs，siRNAs)的方法-2
越來越多的研究人員開始采用小分子干擾RNA（small interfering RNAs，siRNAs)來抑制特定的哺乳動物基因表達。siRNA是一種短片斷雙鏈RNA分子，能夠以同源互補序列的mRNA為靶目標降解特定的 mRNA，這個過程就是RNA干擾途徑（RNA interference
RNAi術語表
RNAi GlossaryDicer?- Dicer is a member of the RNase III family of nucleases that specifically cleave double-stranded RNAs. Dicer processes long dsRNA
Phosphate Assay
1. Make standards using sodium phosphate at the following uM concentrations: 0, 2, 5, 7, 10, 20, 40, 60, and 80. Use the screw top glass tubes.2. Dry
RNAi相關的名詞解釋[英文]
Argonaute?- A family of proteins containing multiple domains and involved in RNA interference (RNAi). Argonatue is the main component of RNAi effector
Fusion and Cloning
Author:?Nanci DonackiSource:?Contributed by Nanci DonackiAbstract:?Procedure for establishing hybridoma in one stepReagents(StemCell Technologies, Inc
Fusion and Cloning
ReagentsMedium A - Pre-fusion Medium and Hybridoma Expansion MediumMedium B - Fusion Medium Medium C - Hybridoma Recovery MediumMedium D - Hybridoma S