WIKI資訊

ISOLATIONOFRNAFROMBACTEROIDS

3 g nodules (fresh or frozen in liquid N2) were ground to a powder in mortar and pestle with liquid N2. To the powder was added ice cold 0.5 M mannitol (or sucrose), 0.05 M Tris (pH 7.5), 0.02 M Na succinate, 5 mM Na dithionite. Pass the resuspension through one layer Miracloth and rinse the cloth with 5 ml of the same buffer solution. Spin filtrate in a sterile centrifuge tube and spin 5 min 5 min at 1500 rpm. Respi......閱讀全文