ImmunofluroescenceTechnique
Fix cells in 2% formaldehyde in PBS/pH 7.4 for 15 min. at 20oC. 2% formaldehyde is made up fresh prior to use by dissolving the appropriate amount of EM grade paraformaldehyde (Prill form, from Electron Microscopy Sciences) in PBS and heating on a hot plate in the hood (setting of 5-6) until the aldehyde goes into solution. Keep the bottle cap loosened so that pressure does not build up. Cool down to 20oC and pH to 7......閱讀全文
Immunofluroescence Technique
Fix cells in 2% formaldehyde in PBS/pH 7.4 for 15 min. at 20oC. 2% formaldehyde is made up fresh prior to use by dissolving the appropriate amount
Sterile Technique
Good sterile technique is the first and most important step in insuring consistent results when employing recombinant DNA and protein expression techn
Principles of Aseptic Technique
INTRODUCTIONThe regulations promulgated to implement the amended Animal Welfare Act require that all survival surgery be performed using aseptic proce
ALKALINE PHOSPHATASE (APAAP) TECHNIQUE
Preparation: Cytological Preparations Fixation: Air dry films or cytospin preparations overnight at room temperature. For frozen and paraffin secti
Principles of Aseptic Technique——2
Personnel Aseptic technique requires careful attention to a series of steps which begins with patient and instrument preparation and ends at final w
Principles of Aseptic Technique——3
PREPARATION OF THE ANIMAL The animals should be prepared in a n area separate from where surgery will be performed. Preparation is facilitated by fi
Principles of Aseptic Technique——3
PREPARATION OF THE ANIMAL The animals should be prepared in a n area separate from where surgery will be performed. Preparation is facilitated by fi
Principles of Aseptic Technique——4
P REPARATION OF THE SURGEONIn a laboratory setting, the extent of surgeon preparation will depend on the facilities and the need for strict attention
Aseptic Technique and Good Cell Culture Practice
Aim To ensure all cell culture procedures are performed to a standard that will prevent contamination from bacteria, fungi and mycoplasma and cross c
沉淀反應技術(Precipitation reaction technique)
一、???? 概述?可溶性抗原（如細菌浸出液、含菌病料浸出液、血清以及其他來源的蛋白質、多糖質、類脂體等）與其相應的抗體相遇后，在電解質參與下，抗原抗體結合形成白色絮狀沉淀，出現白色沉淀線，此種現象稱為沉淀反應。沉淀反應中的抗原叫沉淀原（precipitinogen）,與沉淀原發生反應的抗體稱為沉淀