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Since PCR is often problematic on GC-rich templates, we have evaluated different PCR enhancing additives and generated an Combinatorial Enhancer Solution (CES). We tested this solution on approx. 50 different primer pairs using several different polymerasesThe 5x concentrated Combinatorial Enhancer Solution (CES) contains:2.7 M betaine6.7 mM DTT6.7% DMSO and55 μg/ml BSAAdd this solution to your PCR and see if it work......閱讀全文
Application: Quantitative RT-PCR is used to quantify mRNA in both relative and absolute terms. It can be applied for the quantification of m
ContentsFactors Affecting the PCR Nested Primer PCRPrimer LengthDegenerate PrimersElongation Temperature and TimeReaction BufferCycle Numbe
In the polymerase chain reaction (PCR), a thermostable DNA polymerase amplifies DNA that is flanked by known sequences. The known sequences correspond
Fig. 11. Example of the influence of extension temperature. Multiplex PCR with mixtrues A-B using two different PCR programs. Reactions on the ri
Influence of annealing temperature and number of loci amplifiedLike any other PCR, multiplex reactions should be done at a stringent enough temperatur
Non-denaturing PAA gelsTo separate PCR products differing in only a few bp in length (for example, microsatellite markers), 6-10% PAA gels need to be
實驗概要 We routinely produce dsRNA by in vitro transcription of a PCR generated DNA template containing the T7 promoter se
實驗概要The SuperScript? III One-Step RT-PCR System with Platinum? Taq High Fidelity is designed for sensitive, high-fidelity end-p
利用Mastercycler X50系列PCR獨有的2D梯度實現高效的PCR條件優化Ultimate PCR Optimization with Eppendorf Mastercycler? X50 2D-gradientArora Phang, Tim Schommartz,Eppe
· Standard PCR Protocol (Molecular Biology Techniques Manual)The followings are described in
Figure 5: Regulation of CDH5 by TFZFs in several human cancer cell lines.Blue, cells infected with a pMX construct containing the DNA bindin
A variety of PCR additives and enhancing agents have been used to increase the yield, specificity and consistency of PCR reactions. Whilst these addit
3. Methods3.1 RNA Probe Preparation1. Different strategies can be used to prepare template DNA for synthesizing antisense RNA p
A variety of PCR additives and enhancing agents have been used to increase the yield, specificity and consistency of PCR reactions. Whilst these addit
11. All products in my multiplex reaction are weak. How can I improve the yield?Decrease annealing time in small steps (2o C)Decrease extension temper
Using siRNA for gene silencing is a rapidly evolving tool in molecular biology. There are several methods for preparing siRNA, such as chemical synthe
General AdvicePCR allows the production of more than 10 million copies of a target DNA sequence from only a few molecules. The sensitivity of this tec
General AdvicePCR allows the production of more than 10 million copies of a target DNA sequence from only a few molecules. The sensitivity of this tec
本文來自于哈佛大學醫學院果蠅RNAi篩選中心的經典實驗方法，專門用于果蠅RNAi實驗方法。感謝哈佛大學醫學院果蠅RNAi篩選中心的支持！Primer Designed dsRNATemplate SelectionPCRIn vitro RNA TranscriptiondsRNA Pur
實驗概要We routinely produce dsRNA by in vitro transcription of a PCR generated DNA template containing the T7 promoter sequence on both ends (I. Prim
Fig. 25. Multiplex PCR of mixtures A-D comparing PCR programs with 2 (green) and 1 (yellow) minute extension time at 54° C annealing temperature.
We routinely produce dsRNA by in vitro transcription of a PCR generated DNA template containing the T7 promoter sequence on both ends (I. Primer Desig
Why PrimerBank?PrimerBank is a public resource for PCR primers. These primers are designed for gene expression detection or quantification (real-time
Methylation Specific PCRProtocol written by James Herman*Methylation Specific PCR (MSP) is a simple rapid and inexpensive method to determine the meth
隨著人類基因組計劃（human genome project ）在2003年順利完成，基因組測序技術取得了長足的進步，這直接導致了每兆基因組成本的大幅下降以及檢測的基因組數量越來越多。人們對基因組的復雜性深感震驚，這也引導著測序技術的進一步發展。最近的一些突破性技術使得測序技術在更短的時間內可以
DNA測序（主要內容如下）· Sequencing Gel Preparation· P
實驗概要 Extension of overlapping gene segments by PCR is a simple, versatile technique for site-directed mutagenesis and g
George Church Lab, Harvard Medical SchoolPCR_protocol.html">http://twod.med.harvard.edu/labgc/estep/longPCR_protocol.htmlEfficient Long PCR re
1. DNASIS 2.5 DNASIS for Windows 2.5版是日立軟件公司（Hitachi Sofeware Engineering Co.,Ltd.）97年推出的一個功能強大的序列分析軟件。包含有大部分分子生物學軟件的常用功能，可進行DNA，RNA，蛋白質序列的編輯和分析，
實驗概要The following basic protocol serves as a general guideline and a starting point for any PCR amplification. Optimal reaction conditions