SynapticProteinsattheSynapticJunction
The postsynaptic density (PSD) is a submembranous structure at the postsynaptic membrane mainly at the excitatory synapses. The neurotransmitter receptors are assembled and fixed at the PSD, and several molecules implicated in the synaptic plasticity are also enriched. PSD-95/synapse-associated protein (SAP) 90 is involved in the molecular organization of these components of the PSD and essential for learning and mem......閱讀全文
Synaptic－Proteins－at－the－Synaptic－Junction
The postsynaptic density (PSD) is a submembranous structure at the postsynaptic membrane mainly at the excitatory synapses. The neurotransmitter recep
Blockade－of－Neurotransmitter－Relase－by－Botulinum－Toxin
The neuromuscular junction communicates action potentials from motor neurons across a synapse to skeletal muscle. When an action impulse arrives at th
Purification－of－MBP－(maLTosebinding－proteins)－Fused－Proteins
Express fusion proteins as per the?GST-fused protocol?up to Step 7 (Day 3). All steps in protein purification should be done at 4° C unless otherwise
Purification－of－GST－Fused－Proteins
Day 1Set up an overnight culture in 100 ml LMM broth or 100 ml terrific broth containing 100ul 100 mg/mlAmpDay 2Add 40-50 ml o/n culture to 1 lt terri
Crystallization－of－Kinesin－Family－Motor－Proteins
Motor proteins of several kinesin family groups have now been crystallized: monomeric Kinesin-1 motor domains from human, rat andNeurospora?(Kull et a
Acetylation－(or－Succinylation)－of－Amino－Groups－on－Proteins
Acetylation (or Succinylation) of Amino Groups on ProteinsREFERENCE:?Hanock and Benz. 1986. BBA. 860:699-707.PURPOSE:?Derivitization of amino groups t
Phosphoproteins－pr...
實驗概要The following procedure provides a method of detection of phosphorylated proteins.實驗步驟1.?To a sample of protein solution containing 1-100 ng of
Production－of－Recombinant－Proteins－in－SuspensionCultured－Plant－Cells
Plants have emerged in the past decade as a suitable alternative to the ?current production systems for recombinant pharmaceutical proteins and, ?toda
Bacterial－Expression－of－GSTfusion－Proteins
1.? Grow cells in 5ml (or more) of LB-Amp overnight for a starter culture.?2.? Grow larger culture (100x volume of starter culture) using the overnigh
BTG－family－proteins－and－cell－cycle－regulation
BTG2 is found to be one of the immediate early genes up-regulated by neural growth factor (NGF) and epidermal growth factor (EGF). Its transcriptional
Endocytotic－role－of－NDK,－Phosphins－and－Dynamin
Reliable neurotransmitter release requires the presence of sufficent numbers of synaptic vesicles. The process of synaptic vesicle endocytosis (SVE) i
GJB2基因突變與藥物因子介紹
這個基因編碼縫隙連接蛋白家族的一個成員。間隙連接首先被電子顯微鏡描述為與貼壁細胞接觸的質膜上的區域性特殊結構這些結構是由細胞間通道組成的，這些通道促進了離子和小分子在細胞間的轉移。間隙連接蛋白，也被稱為連接蛋白，從不同組織的富集間隙連接物的餾分中純化。根據核苷酸和氨基酸水平的序列相似性，縫隙連接蛋白
GJB2基因編碼功能及結構描述
這個基因編碼縫隙連接蛋白家族的一個成員。間隙連接首先被電子顯微鏡描述為與貼壁細胞接觸的質膜上的區域性特殊結構這些結構是由細胞間通道組成的，這些通道促進了離子和小分子在細胞間的轉移。間隙連接蛋白，也被稱為連接蛋白，從不同組織的富集間隙連接物的餾分中純化。根據核苷酸和氨基酸水平的序列相似性，縫隙連接蛋白
GJB2基因編碼功能及結構描述
這個基因編碼縫隙連接蛋白家族的一個成員。間隙連接首先被電子顯微鏡描述為與貼壁細胞接觸的質膜上的區域性特殊結構這些結構是由細胞間通道組成的，這些通道促進了離子和小分子在細胞間的轉移。間隙連接蛋白，也被稱為連接蛋白，從不同組織的富集間隙連接物的餾分中純化。根據核苷酸和氨基酸水平的序列相似性，縫隙連接蛋白
TJP1基因編碼的功能和結構描述
該基因編碼膜相關鳥苷酸激酶（maguk）蛋白家族的一個成員，并作為緊密連接銜接蛋白，也調節粘附連接。緊密連接調節內皮細胞和上皮細胞之間離子和大分子的運動。該支架蛋白的多結構域，包括突觸后密度95/盤大/帶狀閉塞區（PDZ）結構域、Src同源結構域（SH3）結構域、鳥苷酸激酶（GuK）結構域和獨特的（
TJP1基因突變因子與藥物介紹
該基因編碼膜相關鳥苷酸激酶（maguk）蛋白家族的一個成員，并作為緊密連接銜接蛋白，也調節粘附連接。緊密連接調節內皮細胞和上皮細胞之間離子和大分子的運動。該支架蛋白的多結構域，包括突觸后密度95/盤大/帶狀閉塞區（PDZ）結構域、Src同源結構域（SH3）結構域、鳥苷酸激酶（GuK）結構域和獨特的（
A－Yeast－Secretion－Trap－Assay－for－Identification－of－Secreted－Proteins－...
Secreted proteins from plants and phytopathogens play important roles in their interactions and contribute to elaborate mechanisms of attack, defe
The－role－of－FYVEfinger－proteins－in－vesicle－transport
Eukaryotic cells take up constituents of the extra-cellular environment and regulate the cell-surface level of membrane proteins via a recycling syste
GProtein－Signaling－Through－Tubby－Proteins
The tubby gene product is expressed in the brain and has been implicated by mouse genetics in obesity and other disorders such as blindness. Structura
Ingel－digestion－of－proteins－for－peptide－fingerprint－mapping
Polyacrylamide gel electrophoresis is a widely used technique to separate proteins from biological samples. Moreover, the development of two-dimension
SemiQuantitative－Measurement－of－Proteins－by－Dot－Blotting
Purpose...Concentration of proteins in a crude preparations (such as culture sup) can be estimated semi-quantitatively by using "Dot Blot" method if y
Isoelectric－Focussing－of－Membrane－Proteins－by－Slab－Gel－Method
REFERENCE:?Ames, G.F.L. and Nikaido, H. 1976. Biochemistry. 15:616-623.MATERIALS:Gel solution:1.05 gacrylamide0.032 gbis-acrylamide8.25 gurea6.5 mldis
PRX基因突變因子與藥物介紹
這個基因編碼一種參與周圍神經髓鞘維持的蛋白質。編碼蛋白包含2個pdz結構域，分別以psd95（突觸后密度蛋白）、dlga（果蠅椎間盤大腫瘤抑制蛋白）和zo1（哺乳動物緊密連接蛋白）命名。已經描述了該基因的兩個選擇性剪接轉錄變體，它們編碼不同的蛋白質亞型，并且在雪旺細胞中具有不同的靶向性。該基因突變可
PRX基因編碼的功能和結構描述
這個基因編碼一種參與周圍神經髓鞘維持的蛋白質。編碼蛋白包含2個pdz結構域，分別以psd95（突觸后密度蛋白）、dlga（果蠅椎間盤大腫瘤抑制蛋白）和zo1（哺乳動物緊密連接蛋白）命名。已經描述了該基因的兩個選擇性剪接轉錄變體，它們編碼不同的蛋白質亞型，并且在雪旺細胞中具有不同的靶向性。該基因突變可
Isolation－of－Cell－Wall－Proteins－from－Medicago－sativa－Stems
Plant cell walls are highly dynamic and chemically active components of plant cells. Cell walls consist primarily of polysaccharides, with protein
Edman－Sequencing－of－Proteins－from－2D－Gels
The Western blotting/sequencing technique using polyvinylidene difluoride (PVDF) membrane is one of the most popular technique for Edman sequencin
目標蛋白(target－proteins)粗提方法4
第四節 提取時的保護性措施提取一些具有生理活性的物質時，除了考慮被提取物溶解度外，還應考慮被提取物活性的保護。對于一些生物大分子如蛋白質、酶和核酸來說，主要措施有如下幾點；1．采用緩沖系統 防止提取過程中某些酸堿基團的解離，造成溶液中PH值變化幅度過大，導致某些活性物質的變性、或由于PH變化影響
Phosphoamino－acid－analysis－of－nonTCA－precipitable－proteins
Phosphoamino acid analysis of non-TCA precipitable proteins1. Cut out gel slice, incubate 60 min with 1 ml 5.7 M HCl @ 110oC in 13 x 100 mm screw cap
Preparation－of－Bacterial－Proteins－for－Analysis－by－2DPAGE
The following protocol has been developed for preparing soluble bacterial proteins in a form suitable for analysis by 2D-PAGE. The procedure was princ
目標蛋白(target－proteins)粗提方法3
3）pH值 一般在被提取蛋白質的等電點的兩側，堿性蛋白用稀酸提取、酸性蛋白用稀堿提取。在某些情況下，為了破壞所分離的蛋白質與其他雜質的靜電結合，選擇偏酸（pH3-6）或偏堿（pH10-11），可以使離子鍵破壞而獲得單一的蛋白成分。4）溫度 提取時通常要求低溫操作。只有對某些耐高溫的蛋白質或酶（如胃蛋