BiosynthesisofProlineinBacteria
Among the twenty amino acids encoded by the genetic code, proline is unique in having a cyclic structure with its side chain connected to the amino group to create a secondary amine. As a consequence of its cyclic structure, proline constrains the structure of proteins where it occurs, disrupting alpha-helices. Isomerization between the cis and trans forms of proline in proteins is isomerized by peptidyl-prolyl isome......閱讀全文
Biosynthesis－of－Proline－in－Bacteria
Among the twenty amino acids encoded by the genetic code, proline is unique in having a cyclic structure with its side chain connected to the amino gr
Biosynthesis－of－Tryptophan－in－Bacteria－and－Plants
The aromatic amino acid tryptophan is an essential nutrient, meaning that humans and animals do not themselves have the biosynthetic machinery to synt
Biosynthesis－of－Chorismate－in－Bacteria－and－Plants
The biosynthesis of all three aromatic amino acids (tryptophan, tyrosine and phenylalanine) begins with the metabolic intermediate chorismate. The bio
Biosynthesis－of－Cysteine－from－serine－in－bacteria－and－plants
In animals cysteine is synthesized from homocysteine, a produce of the essential amino acid methionine. In the absence of dietary methionine, animals
Bacteria－Growth－and－Culture－Bacteria－Growth－and－Culture
Flagella and motilitymonotrichous?flagella - the bacterial cell has a single flagellaperitrichous?flagella - the bacterial cell has several flagella w
Biosynthesis－of－neurotransmitters
Neurotransmitters are signaling molecules used by neurons to communicate across chemical synapses. The receptors for neurotransmitters include G-prote
Biosynthesis－of－isoleucine
Isoleucine is an essential amino acid, only synthesized in plants and bacteria, and required in the diet by animals. In proteins, the hydrophobic isol
Biosynthesis－and－Analysis－of－Bilins
The term bilin is a collective one to describe a broad group of open chain tetrapyrroles and derives from the name “bile pigments” as the first o
Biosynthesis－of－threonine－and－methionine
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Phosphatidylcholine－Biosynthesis－Pathway
The main components of biological membranes are phosphoglyceride lipids composed of a glycerol unit esterified to two fatty acids and a polar alcohol
Biosynthesis－of－Glycine－and－Serine
Intermediates in energy production pathways such as glycolysis and the Kreb's cycle are commonly the starting point for the biosynthesis of amino
Biosynthesis－of－Cysteine－in－mammals
In animals, the sulfur-containing amino acid cysteine is synthesized from homocysteine, which is itself derived from metabolism of the amino acid meth
Biosynthesis－of－spermidine－and－spermine
The polyamines spermidine and spermine are expressed in a variety of tissues and are involved in the regulation of apoptosis, cellular proliferation,
Testing－for－Bacteria－and－Fungi
AimIn cases of gross contamination the naked eye may identify the presence of bacteria and fungi. However, it is necessary to detect low-level infecti
Bacteria－Culture－Protocol
Bacteria Culture ProtocolBy 徐曉政1、TBS Medium Preparation:Prepare 1L of TBS medium contains:Tryptone 12gYeast extract 24gNaCl 5gSodium Succinate 5gGlyce
Streaking－Bacteria－for－Single－Colonies
1. Initial inoculum:- From solid media, touch an isolated colonies with a sterile applicator or toothpick.- From liquid media, touch the culture with
Isolation－of－genomic－DNA－from－bacteria
Note: This procedure does not work well with Gram + cocci. Transfer 1.5 mL overnight culture to a 1.5 mL microfuge tube, centrifuge for 30 sec, de
Agar－Plates－for－Selection－of－Clones－in－Bacteria
Cloning of PCR products Stocks: LB Agar: Luria Broth after Lennox: per Liter Tryptone 10 g Yeast Extract 5 g Sodium Chlori
Bacteria,－cont.－Growth－and－Reproduction
Flagella and motility monotrichous?flagella - the bacterial cell has a single flagella peritrichous?flagella - the bacterial cell has several f
Catabolic－Pathways－for－Arginine－－Histidine－Glutamate－Glutamine,－and－Proline
Glutamine is converted to glutamate by glutaminase or several other enzymes by the removal of the amide nitrogen. Proline is first converted to a Schi
Proline－Plus家族又添新成員
Proline Plus家族有新成員啦！?百得（Biohit）Proline Plus手動移液器家族添加新成員啦Proline Plus 5-50μl單道移液器將成為整支高溫高壓消毒系列最理想的投標產品。????????????? 目前Proline Plus成為百得（Biohit）
Phospholipid－Biosynthesis－in－E.－Coli－Pathway
The biosynthesis of membrane phospholipids occurs through distinct pathways in mammals and bacteria. In the mammalian pathway for the synthesis of pho
Maintenance－of－Probes－in－bacteria－including－Escherichia－coli
Plasmid (pUC series) containing genomic DNA fragments are maintained in E. coli strain DH5aTM. The E. coli cultures are routinely cultured at 37 C on
Probiotic－Attributes－of－Lactic－Acid－Bacteria－Isolates
IntroductionPresently the consumption of probiotic food is highly popular worldwide for their health beneficial effects. Commonly used probiotic bacte