FreeRadicalInducedApoptosis
Oxidative stress is one factor that can trigger programmed cell death. Activated neutrophils responding to inflammatory stimulation produce reactive oxygen species like superoxide free radicals to kill invading bacteria, but these reactive oxygen species can also attack endothelial cells lining the vascular wall and trigger apoptosis. Endothelial cells also produce reactive oxygen species inside the cell that can con......閱讀全文
A rapid, quantitative and inexpensive method for detecting apoptosis
ASTRACTWe describe a rapid and quantitative flow cytometric method for determining the apoptotic or anti-apoptotic potential of a gene in various cell
PTEN dependent cell cycle arrest and apoptosis
PTEN is a tumor suppressor gene. Recombinant PTEN is capable of dephosphorylating phosphatidylinositol 3,4,5-triphosphate[PI(3,4,5)P3], the product of
Role of nicotinic acetylcholine receptors in the regulation of apoptosis
Nicotinic acetylcholine receptors are essential for neuromuscular signaling and are also expressed in non-neuronal tissues, where their function is le
細胞凋亡（apoptosis）的檢測-2
【材料】１試劑：吖啶橙貯存液（10mg吖啶橙溶解于100mLPBS中，過濾，4℃避光保存），0.01mol/L、Ph6.8PBS。２器材：吸管，試管，玻片，熒光顯微鏡。【方法】１制備待檢活細胞懸液，濃度約為1×10７/mL。２取95uL的細胞懸液，加5uL的吖啶橙貯存液混勻。３吸一滴混合液
細胞凋亡（apoptosis）的檢測-1
細胞凋亡（apoptosis）是一種由基因控制的細胞自主性死亡方式。其發生誘因及形態學特征均有別于壞死，與人類免疫系統的發生發展、神經組織發育、器官的形成、腫瘤的發生發展等諸多生物學現象之間有著密切聯系，并且是免疫應答過程中免疫細胞的殺傷機制之一。細胞凋亡的檢測技術已成為免疫學檢測的一個重要內容。凋
細胞凋亡（apoptosis）的檢測-3
【結果判斷】細胞出現程序化細胞死亡時，在瓊脂糖凝膠電泳帶上呈現有一定間隔的梯狀條帶，而細胞壞死時則呈模糊的片狀條帶（無間隔），陰性對照者僅在近電泳點樣處出現基因組條帶。（3）流式細胞儀觀察－PI染色法【原理及意義】細胞凋亡時，流式細胞檢測可呈現亞二倍體核型峰的特征。此外，根據細胞光散射特點，應用碘化
Opposing roles of AIF in Apoptosis and Cell Survival
Programmed cell death is induced by many different factors and involves numerous signaling pathways, some dependent on caspase proteases and others th
Cell-free System for the examination of apoptotic activity
?IntroductionIn our lab we use the term 'cell-free system' when we talk about the examination of apoptotic activity in cytoplasmic extracts. T
Xeno-free Culture of Neural Stem Cells
實驗概要Neural ?stem cells (NSCs) derived from human embryonic stem cells (hESCs) have ?the potential to help provide understanding for human neurogenesis
Simultaneous analysis of DNA content
Simultaneous analysis of DNA content and surface immunophenotype using gentle ethanol fixation techniques.??William Telford. Louis E. King and Pamela
XIAP基因編碼的功能和結構描述
該基因編碼一種屬于凋亡抑制蛋白家族的蛋白質。這個家族的成員有一個保守的基序，叫做桿狀病毒iap重復序列，這對他們的抗凋亡功能是必要的。該蛋白通過與腫瘤壞死因子受體相關因子traf1和traf2結合發揮作用，并抑制自由基誘導劑甲萘醌和白細胞介素1β轉化酶誘導的細胞凋亡。這種蛋白質還抑制至少兩種細胞死亡
XIAP基因突變因子與藥物介紹
該基因編碼一種屬于凋亡抑制蛋白家族的蛋白質。這個家族的成員有一個保守的基序，叫做桿狀病毒iap重復序列，這對他們的抗凋亡功能是必要的。該蛋白通過與腫瘤壞死因子受體相關因子traf1和traf2結合發揮作用，并抑制自由基誘導劑甲萘醌和白細胞介素1β轉化酶誘導的細胞凋亡。這種蛋白質還抑制至少兩種細胞死亡
An Integrative Procedure for Apoptosis Identification and Measurement-2
TroubleshootingCritical Steps(1) Don’t trypsinize cells for too long when collecting them.(2) Rotation speed should be no more than 1500 rpm during ce
Apoptosis: A Laboratory Manual of Experimental Methods Andrea Cossarizza
THE CELL?1.?Morphological aspects of apoptosis?Walter Malorni, Stefano Fais & Carla Fiorentini?2.?Cell cycle?Miriam Capri & Daniela BarbieriTHE NUCLEU
In Situ Cell Death (Apoptosis) Detection by TUNEL labeling
Protocol for Frozen Sections:Warm 150ml 4% Paraformaldehyde/1x PBS to RT. Fix slides in it, 20 min., RT.1x PBS rinse, 2 times.1x PBS, 30 min., RT. Beg
Detection Of Cell Viability And/Or Apoptosis By Flow Cytometry (FACS)
Viable?cells are cells that when allowed to continue beyond the timepoint of examination will stay alive. Besides live and healthy cells, cells in ear
細胞凋亡（Apoptosis）檢測技術與方法
細胞凋亡（Apoptosis），又稱細胞程序性死亡（PCD： Programmed Cell Death），是指細胞在一定的生理或病理條件下，遵循自身的程序，自己結束其生命的過程。它是一個主動的，高度有序的，基因控制的，一系列酶參與的過程。細胞凋亡概念提出至今還不到30年的時間，但由于它在保證多
自旋標記法的方法介紹
自旋標記 （spin label）， 很多物質的分子不表現電子自旋共振（ESR），但對這些分子，人工地使之與自由基（free radical）結合從而得以用ESR法來研究，獲得獨特的ESR信息，這就是自旋標記法。
Preparation of cytoplasmic extracts for the application inacell-free system
Characteristics of this procedure: Cells are grown to 80% confluency, then harvested, washed and disrupted in KPM buffer by freezing-thawing cycl
Miniprep/Kit-free high-throughput protocol
Background This protocol is adapted from "Molecular Cloning: A Laboratory Manual", Second Edition, Sambrook, Fritsch, and Maniatis. It is a quick, i
Isolation of cell nuclei for the application in the cell-free system
Characteristics of the procedurePreparation of isolated nuclei - procedurePreparation of radioactive labeled nucleiMaterial?Characteristics of the pro
Free β-HCG提升唐氏癥篩檢率
隨著孕婦年齡的增加，生出唐氏癥兒的機率也隨之增加。據 Hook 的統計，20 歲的產婦生下唐氏癥兒的機率為 1/1923，30 歲時機率為 1/885，35 歲為 1/365，40 歲則為1/109（1）。因此母親年齡也可用來篩檢唐氏癥，通常建議生產時的實足年齡超過 35 歲應該要做羊膜穿刺術。雖然
CBL mediated ligand-induced downregulation of EGF receptors
As with many cell-surface receptors, activation of the EGF receptor can result in receptor internalization through receptor-mediated endocytosis, dese
Lipoprotein Analysis Week 2: Electrophoresis
Lipoprotein Analysis??Week 2: Electrophoresis?IntroductionSDS polyacrylamide gel electrophoresis (SDS PAGE) will be used to assess the purification pr
Role of MEF2D in T-cell Apoptosis
Mef2 (Myocyte enhancer factor 2) transcription factors play a role in T-Cell Calcium Induced Apoptosis. Several factors regulate Mef2 transcription fa
中山大學最新《Blood》文章解析細胞因子新發現
鄭利民 教授 1984年畢業于上海醫科大學后在附屬華山醫院任臨床醫生，94年獲荷蘭萊頓（Leiden）大學細胞生物和免疫學博士學位，1999年獲瑞典國家研究院人才基金（助理教授）資助而在瑞典林雪平大學建立了獨立的實驗室。2002年被聘為中山大學教授，博士導師。現任中山大學生命科學院生物化學系主
Neuropeptides VIP and PACAP inhibit the apoptosis of activated T cells
Vasoactive intestinal peptide (VIP) and the structurally related pituitary adenylate cyclase-activating polypeptide (PACAP), two neuropeptides present
ASENSITIVE METHOD FOR DETECTION OF APOPTOSIS BY SINGLE LASER FLOW CYTOMETRY
MATERIALS:1. 1 X PBS (PBSAz, 1 X PBS, e.g., Irvine Scientific, CA, containing 2% newborn calf serum and 0.1% sodium azide)2. 7-Amino-actinomycin D (7-
A rapid, quantitative and inexpensive method for detecting apoptosis-2
Figure?1.Determination of apoptosis in transiently transfected murine [beta] tumor cells. (A) The number of apoptotic [beta]HC 13T tumor cells (% apop