WIKI資訊
Use of Semi-Thin Cryosections for Light Microscopy.Semi-thin sections can be obtained from frozen blocks of cryoprotected biological material by sectioning at -90°C. The advantages for using these sections for light microscopy are that the subcellullar morphology is preserved and the optical resolution is improved. Chemically fixed biological material is cryoprotected by immersion in 2.3 M sucrose and frozen onto spe......閱讀全文
INTRODUCTIONThis protocol describes the histological preparation of embryonic and adult zebrafish eyes. The methods described here can be easily adapt