Chorioallantoicmembranegraftingwithchickembryolimbbuds
ObjectiveThis experiment explores the ability of the chick chorioallantoic membrane (CAM) to support an excised limb bud from a donor embryo. The chick system will allow observation of general cartilage formation in limb grafts on the chorioallantoic membrane and to study the role of the CAM in calcium transport in ovo. IntroductionExtraembryonic membranes regulate crucial functions in the chick egg, including w......閱讀全文
Chorioallantoic－membrane－grafting－with－chick－embryo－limb－buds
ObjectiveThis experiment explores the ability of the chick chorioallantoic membrane (CAM) to support an excised limb bud from a donor embryo. The chic
Joint－formation－in－chick－limb－bud－CAM－grafts
Abstract????????Choriallantoic membrane (CAM) limb grafting functions as a method to isolate the inductive events of limb formation. Experimenters hav
Chick－Chorioallantoic－Membrane－(CAM)－Assay
CAM ASSAYShell-less embryo cultureFertilized white leghorn chicken eggs (SPAFAS Inc., Norwich, CT) were received at day 0 andincubated for 3 days at 3
Embryonic－limb－bud－culture－in－media
Early in embryonic development, the region of the chick embryo which is determined to form a limb first differentiates from the rest of the embryo1. T
Chick－Embryo－staging
In order to repeat a published experiment, or have someone else repeat yours, it is important to use the same materials. For developmental studies, t
FGFR－INHIBITOR,－SU5402,－ON－CRANIOFACIAL－DEVELOPMENT－IN－CHICKEN－EMBRYOS
ABSTRACTFibroblast growth factor (FGF) signaling plays a signifigant role in embryonic development. Particularyl, FGFs are implicated in mediating epi
Chorioallantoic－Membrane－(CAM)－Assay
8 eggs per day, day 7- day 13?cut CAM, wash in precooled PBS,in 10 ml WASH 1 (PBS, 5 mM EDTA, COMPLETE) on icecut in pieces in petri dish on icecentri
Exercise－12.10－－Establishment－of－a－Primary－Culture
Exercise 12.10 - Establishment of a Primary Culture LEVEL III Materials Chick embryo (approximately 8 days old) 70% (v/v) ethanol for swa
Cytokine－induced－angiogenesis－in－chick－embryos.
Objective:The purpose of this experiment was to explore the effects of the growth factors bFGF and VEGF on blood vessel formation within the chorioall
Observation－of－living－and－plasticembedded－chick－embryos
The development of chick embryos has been studied since Aristotle. It is one of the most intensely studied organisms. One reason for this is that ther
Shellless－cultures－of－chick－embryos
This experiment allows you to observe the development of a chick embryo outside its shell. Cultured chicks are also more accessible for manipulation.
In－VitroCulture－of－Chicken－Heart
The chicken is a classic organism used to illustrate the principles of basic embryology. One of the developmental systems which has been examined in g
Effect－of－Cyclopamine－on－Xenopus－laevis－embryos
Cyclopamine and jervine, teratogens derived from the skunk cabbage?Veratrum californicum, are known to induce holoprosencephaly in chick embryos when
The－Developmental－Effects－of－Nicotine－on－Chick－Embryos
More than 22 million American women smoke and approximately 25% of these women continue to smoke during pregnancy (Lambers and Clark 1996). Cigarette
ORGANOTYPIC－LIMB－CULTURES
-embryos are dissected from timed-pregnant mice from 10.5 - 11.5 d.p.c.-limb buds are microdissected and placed in holding medium (L15 medium suppleme
Determining－the－effects－of－nicotine－on－bone－－in－chicks
ObjectiveThe purpose of this experiment is to determine how the development of bone and cartilage in a chick are affected by treating the embryo with
Zebrafish－whole－mou...
實驗概要Whole mount staining of Zebrafish embryos, now commonly used, requires extra steps to fix and permeabilize to ensure the egg membrane is perme
Chick－or－Mouse－embr...
實驗概要The following procedure describes the procedure for whole mount staining of chick or mouse embryo’s. A similar procedure could be used for sta
Developmental－and－phenotypic－implications－of－transplantation
ObjectiveIn this experiment, a region of the neural crest will be transplanted from one stage-fourteen embryo to another (Figure 1). To facilitate the
Whole－mount－fluores...
實驗概要The method ?provides a protocol for whole mount fluorescent immunohistochemistry. ?The advantage of using fluorescence to stain whole mount sectio
Embryo－Lysates－－Immunoprecipitation
Embryo lysatesTake 25 embryos and place into 1.7ml centrifuge tube.Rinse once in lysis buffer (add ~ 1ml) and remove by aspirationAdd 500 μL lysis buf
Regulative－Development－in－Axolotl－Embryos;－Splitting－the－Heart－Field
ObjectiveThis experiment will seek to demonstrate regulative development in?Ambystoma mexicanum?embryos. Specifically, we will split the morphogenetic
Development－of－the－Heart－Morphogenetic－Field－in－the－Axolotl－Embryo
IntroductionThere are two modes of development common to most species in the animal kingdom.Virtually all embryos undergo both mosaic and regulative d
Immature－Embryo－Rescue－and－Culture
Embryo culture techniques have many significant applications in plant breeding, as well as basic studies in physiology and biochemistry. Immature
MEDIA－FOR－EMBRYO－CULTURE－AND－MANIPULATION
M16 Medium (Protocol obtained from Karen Austen-Reed from SS Tan Laboratory, Anatomy Department) For oocyte maturation and routine culture of mouse
Embryo－Dissection－and－Micromanipulation－Tools
Embryo Dissection and Micromanipulation ToolsHazel L. Sive,?Robert M. Grainger, and?Richard M. HarlandAdapted from "Equipment for Embryo Experiments,"
DGK－Membrane－Preparation
Reagents: Bacterial strain E.?coli N4830/pJW10 LB amp media 50 μg/ml ampicillin High salt buffer for 1 L 50 mM KH2PO4 6.8 g 150 mM KCl 11.18 g 50
CELL－MEMBRANE－PREPARATION
I.? Solutions:?A.? Ca and Mg free Phosphate Buffered Saline (PBS) solution,?? buffered with 0.02M Hepes.? pH=7.4?B.? Ca and Mg free PBS, buffered with
The－Effect－of－Caffeine－on－the－Heart－Rates－of－ThreeDay－Old－Chick－Embryos
Objective??????The purpose of this experiment is to observe the effect of caffeine on the heart rate of three day old chick embryos by directly treati
In－vitroculture－of－early－chick－embryos
1. Use sterile technique. Prewarm Howard's Ringers in petri dish and agar/albumin culture dish to 37oC. 2. Crack 2-day egg into large sterile pe