GenomeWideIdentificationofTranscriptionFactorBindingSitesin...
Genome-Wide Identification of Transcription Factor-Binding Sites in Plants Using Chromatin Immunoprecipitation Followed by Microarray (ChIP-chip) or Sequencing (ChIP-seq)Nearly all signal transduction pathways lead to regulation of gene expression by controlling specific transcription factors (TFs). Chromatin immunoprecipitation (ChIP) is a powerful method for studying TF–DNA interactions in vivo. To identi......閱讀全文
Genome-Wide Identification of Transcription Factor-Binding Sites in...
Genome-Wide Identification of Transcription Factor-Binding Sites in Plants Using Chromatin Immunoprecipitation Followed by Microarray (ChIP-chip)
軟件:Genome-wide Association Study Software
The software included are listed bellow ? IMPUTE a program for genotype imputation in genome-wide association studies and fine-mapping? studies
An Integrative Procedure for Apoptosis Identification and Measurement
IntroductionApoptosis is a normal physiological phenomenon put forward by Kerr [1]. It plays an important role in embryonic development, maintenance o
a pipeline for the identification of intact N-glycopeptides(一)
pGlyco: a pipeline for the identification of intact N-glycopeptides by using HCDand CID-MS/MS and MS3 ? Wen-Feng Zeng1,2,*, Ming-Qi Liu3,*, Yang Zh
a pipeline for the identification of intact N-glycopeptides(二)
Methods ? Materials. A uniform mixture of six standard glycoproteins was used as the starting material, including IgG (56834, catalog numeber),
a pipeline for the identification of intact N-glycopeptides(五)
Alignment between MS/MS and MS3 identifications. Glycans were identified after analyzing the HCD/CID-MS/MS spectrum pairs, but the sequences of p
a pipeline for the identification of intact N-glycopeptides(六)
? ? Figure 4. Comparison of different decoy strategies. The estimated FDR of each decoy method is compared with the real FDR. “Decoy: + 11” m
An Integrative Procedure for Apoptosis Identification and Measurement-2
TroubleshootingCritical Steps(1) Don’t trypsinize cells for too long when collecting them.(2) Rotation speed should be no more than 1500 rpm during ce
a pipeline for the identification of intact N-glycopeptides(七)
Complementary ion information provided by HCD- and CID-MS/MS. Both HCD- and CID-MS/MScould be used to optimize the glycopeptide identification. Rece
a pipeline for the identification of intact N-glycopeptides(三)
Figure 1. The overall workflow of pGlyco. First the sample is analyzed by HCD-MS/MS (NCE = 40%).Then the product-dependent CID-MS/MS and data-depend