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This chapter describes a detailed protocol for genomic Southern blot analysis which can be used to detect transgene or endogenous gene sequences in cereal genomes. The protocol follows a standard approach that has been shown to generate high-quality results: size fractionation of genomic DNA; capillary transfer to a nylon membrane; hybridization with a digoxigenin-labelled probe; and detection using a che......閱讀全文
The influence of methylation on the promoter activity and gene expression and the involvement of DNA methylation in carcinogenesis caused an extensive
Southern雜交One important thing for transfer:the weight of the object resting on top of the blotting apparatus should not exceed the weight equal to a 5
DNA PreparationGene TransferEmbryo TransferTransgenic IdentificatioinOthersTransgenic Outline (University of Michigan Transgenic Animal Model Cor
實驗概要DNAzol? Reagent (Genomic DNA Isolation Reagent) is a complete and ready-to-use reagent for the isolation of genomic DNA from solid and
分子診斷技術是指以DNA和RNA為診斷材料，用分子生物學技術通過檢測基因的存在、缺陷或表達異常，從而對人體狀態和疾病作出診斷的技術。其基本原理是檢測DNA或RNA的結構是否變化、量的多少及表達功能是否異常，以確定受檢者有無基因水平的異常變化，對疾病的預防、預測、診斷、治療和預后具有重要意義。通俗
Methylated CpG island Amplification Protocol written by Minoru Toyota2. Materials2.1. MCARestriction enzymes SmaI, XmaIT4 DNA ligaseTaq DNA polym
實驗概要Plant DNAzol? is an extra-strength-DNAzol? reagent (patent pending) specifically formulated for the isolation of genomic DNA from plants. The Plan
1.RNAi ：（RNA interference）RNA干擾一些小的雙鏈RNA可以高效、特異的阻斷體內特定基因表達，促使mRNA降解，誘使細胞表現出特定基因缺失的表型，稱為RNA干擾（RNA interference，RNAi ，也譯作RNA干預或者干涉）。它也是體內抵御外在感
For an in-depth review of the method, see Friedman, K. and B. Brewer (1995) Analysis of replication intermediates by two-dimensional agarose g
For an in-depth review of the method, see Friedman, K. and B. Brewer (1995) Analysis of replication intermediates by two-dimensional agarose gel elect
We have found that the DNA extraction procedure of Metzenberg and Baitch (Neurospora Newsl. 28:20)/Stevens and Metzenberg (Neurospora Newsl. 29:27) wh
Differential cDNA Screening ProceduresThe protocols listed refer to cDNA library construction and preliminary differential screening procedures. They
Molecular biology experiments often require preparation of small amounts of DNA from many samples. This abbreviated DNA isolation method yields an ave
Picking ES cell clonesOne or two days before picking colonies prepare 24-well plates of feeders. You can also use alternate protocols that utilize 96-
說到基因編輯，大家都會想起CRISPR技術。這個當年名聲大噪的技術，現今依舊熱度不減，尤其是我們的CRISPR大神張鋒，近期發表的文章頻頻亮相于知名雜志，又引起一片熱議。時過境遷，CRISPR技術并沒有銷聲匿跡，一直在線。但任何事物包括技術有利就有弊，CRISPR技術當然也毫不例外。CRISPR技術
分子診斷技術是指以DNA和RNA為診斷材料，用分子生物學技術通過檢測基因的存在、缺陷或表達異常，從而對人體狀態和疾病作出診斷的技術。其基本原理是檢測DNA或RNA的結構是否變化、量的多少及表達功能是否異常，以確定受檢者有無基因水平的異常變化，對疾病的預防、預測、診斷、治療和預后具有重要意義。通俗
Fine Mapping of Genomic Targets of Nuclear Proteins in Cultured CellsAchim Breiling and Valerio OrlandoDulbecco Telethon Institute, Institute of Genet
一、核酸分子雜交技術1961年Hall開拓了液相核酸雜交技術的研究，其基本原理是利用核酸分子單鏈之間有互補的堿基順序，通過堿基對之間非共價鍵的形成，出現穩定的雙鏈區，形成雜交的雙鏈。自此以后，由于分子生物學技術的迅猛發展，特別是70年代末到80年代初，分子克隆、質粒和噬菌體DNA的構建成功，核酸自動
一、核酸分子雜交技術1961年Hall開拓了液相核酸雜交技術的研究，其基本原理是利用核酸分子單鏈之間有互補的堿基順序，通過堿基對之間非共價鍵的形成，出現穩定的雙鏈區，形成雜交的雙鏈。自此以后，由于分子生物學技術的迅猛發展，特別是70年代末到80年代初，分子克隆、質粒和噬菌體DNA的構建成功，核酸自動
1. Run the gel as normal. Often for genomic southerns it is desirable to run long gels (18cm) over 4-6hrs.2. Photograph the gel with a ruler adjacent
Megabase DNA IsolationMegabase-size DNA isolation from plantsTo construct large insert DNA libraries in BAC and YAC vectors, methods must be developed
· In Vitro Mutagenesis Using Altered Sites (Bowtell Lab) In vitro Mutagenesis with dut
實驗概要The E.Z.N.A.? Tissue DNA Kit provides a rapid and easy method for the isolation of genomic DNA for consistent PCR and Southern analysi
實驗概要The iPrep? GeneCatcher? gDNA Blood Kit allows rapid and automated extraction of genomic DNA (gDNA) from human blood including archived
HYBRIDIZATION.Prehybridize blot at 65oC for ~3h in Church buffer containing 0.5mg/ml denature salmon sperm DNA (usually 14ml Church buffer plus 0.7ml
Yeast DNA PreparationYeast Genomic Preparation (Gottschling Lab)Rapid method for yeast genomic DNA isolation Yeast DNA Preparation (r
實驗概要The E.Z.N.A.? Tissue DNA Kit provides a rapid and easy method for the isolation of genomic DNA for consistent PCR and Southern analysis. Up to
Why PrimerBank?PrimerBank is a public resource for PCR primers. These primers are designed for gene expression detection or quantification (real-time
實驗概要The E.Z.N.A.? Tissue DNA Kit provides a rapid and easy method for the isolation of genomic DNA for consistent PCR and Southern analysis. Up to
mRNA差異顯示技術（differential display，DD）是用于研究基因的差異表達的新方法。該技術自1992年被首次報道后，即以其不可替代的優勢被廣泛應用于生物醫學領域。在應用過程中不斷得到改進，并產生了諸多衍生技術如RPA（RNA finger printing by arbitrar