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基于epMotion5075t及KAPA文庫定量試劑盒的全自動NGS文庫...2

Results and DiscussionAutomated qPCR Setup Accuracy and PrecisionThe accuracy and precision of the qPCR setup was evalu ated based on standard curve analysis (Figure 3). Amplifca tion efciency is equivalent between automated (95%) and manual (94%) setup, and both values are within the acceptable range of 90 – 110%. The coefcient of variation (R2) obtained for the two liquid handling methods is similar with a ......閱讀全文