Comparativeassessmentofglycosylationofrecombinanthuman...（七）
Figure 3 MS2 spectra of 2-AB-labeled N-glycan structures. Diagnostic ions are marked with corresponding fragment structures. (a) NeuGc1NeuAc1HexNAc4Hex5 from rhFSH [(M+2H)2+ at m/z 1180.34]. (b) NeuAc2HexNAc4Hex5SO41 from rhFSH, [(M+Na+H)2+ at m/z 1214.36]. *The NeuAc was dehydrated. (c) and (d) The two isomers of Fuc2NeuAc1HexNAc5Hex5 from uhFSH, [(M+2H)2+ at m/z 1274.48]. Figure 4 Site-specific chara......閱讀全文
Comparative－assessment－of－glycosylation－of－recombinant－human－...（七）
Figure 3 MS2 spectra of 2-AB-labeled N-glycan structures. Diagnostic ions are marked with corresponding fragment structures. (a) NeuGc1NeuAc1HexNA
Comparative－assessment－of－glycosylation－of－recombinant－human－...（三）
LC-MS data processingN-glycan data were processed using UNIFI 1.7 with Glycobase 3+ (Waters Corporation, Milford, MA) for N-glycan structure. The pe
Comparative－assessment－of－glycosylation－of－recombinant－human－...（五）
Supporting Information(1) RP-UPLC-Q-TOF analysis of hFSHs subunits; (2) molecular weights and possible glycan structures of hFSHs subunits; (3) pr
Comparative－assessment－of－glycosylation－of－recombinant－human－...（一）
Comparative assessment of glycosylation of recombinant human FSH and highly purified FSHHong Wang, Xi Chen, Xiaoxi Zhang, Wei Zhang, Yan Li, Hongrui Y
Comparative－assessment－of－glycosylation－of－recombinant－human－...（四）
Site-specific characterization of N-glycansFor intact N-glycopeptide analysis, chymotryptic digests of both hFSHs were subjected under UPLC equipped
Comparative－assessment－of－glycosylation－of－recombinant－human－...（二）
Experimental sectionChemicals and reagentsOne lot of PuregonR-HP of 50 IU/0.5mL and two lots of PuregonR-HP of 100 IU/0.5mL (rhFSH) (Organon, Oss, N
Comparative－assessment－of－glycosylation－of－recombinant－human－...（六）
(22) Wu, S. W.; Pu, T. H.; Viner, R.; Khoo, K. H. Novel LC-MS/MS product dependent parallel data acquisition function and data analysis workflow f
Synthesis－of－Recombinant－Human－Cytokine－GMCSF－in－the－－Seeds－of－Transge...
We are interested in studying plant systems as vehicles for the production of recombinant proteins of clinical relevance. There are a number of po
重組人表皮細胞生長因子（recombinant－human－EGF）
生物學功能：人表皮細胞生長因子（hEGF）不僅對上皮細胞而且對許多類型細胞均有促增殖作用，是與細胞的生長及分化有關的重要生長因子。hEGF具有多種生物活性，主要表現在：①在體內刺激皮膚組織、角膜和氣管上皮組織的生長繁殖； ②加速角膜、皮膚等表皮創傷的修復； ③抑制胃酸分泌；④促進人皮膚上皮細胞、角膜
Comprehensive－identification－of－novel－proteins－and－Nglycosylation－sites七
Data analysisTandem mass spectra were retrieved using Xcalibur (version 2.2, Thermo Fisher Scientific) and AnalystTF (version 1.6, AB SCIEX) softw
流式應用精選——微核檢測（體內體外）
?注：點擊文章名稱查看詳情。文章名稱樣本儀器型號備注Assessment?of?the?Genotoxic?Potential?of?Azidothymidine?in?the?Comet,Micronucleus,?and?Pig-a?AssayBone?Marrow?and?Peripheral
高通量測序技術在腸道菌群多樣性研究中的應用（三）
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CO2恒溫搖床解決人胚腎－293－(HEK293)－細胞結團問題（一）
人胚腎 293 (HEK293)? 細胞在重組蛋白表達中是最常見的宿主細胞。 這類細胞能夠表達大量的膜蛋白，如 G 蛋白偶聯受體? (GPCR) ，是無法在最常見的生物制藥生產宿主，如：中國倉鼠卵巢 (CHO) 細胞中作表達。 HEK293 雖然是蛋白表達的極好宿主，然而 HEK293 細胞
In－vitro－Assessment－of－Metabolic－－in－Suspension－Cryopreserved－Hepatocytes
實驗概要BackgroundThe ?pharmaceutical and biotechnology industry’s goal is to discover ?therapeutic agents that are both safe and effective at treating or
Glycosylation－of－Antibody－Therapeutics:－Optimisation－for－Purpose
Recombinant antibody therapeutics represent a significant success story in terms of clinical benefit delivered and revenue (profit) generated with
Assessment－of－Magnaporthe－grisea－mating－type－by－spore－PCR
Isolation of DNA from filamentous fungi for PCR analysis is usually time consuming and involves use of toxic chemicals such as phenol/chloroform. In?S
Comprehensive－identification－of－novel－proteins－and－Nglycosylation－sites九
41. Schmidt O, Theopold U, Strand M: Innate immunity and its evasion and suppression by hymenopteran endoparasitoids. BioEssays 2001, 23(4):344–35
歐盟評估一種轉基因大豆作為食品和飼料的安全性
2019年11月11日，歐盟食品安全局（EFSA）發布消息，歐盟食品安全局轉基因生物小組（GMO Panel）評估了轉基因大豆MON 87751×MON 87701×MON 87708×MON 89788用作食品和飼料的安全性。 經過評估，GMO小組得出結論，就對人類和動物健康以及環境的潛在影
Production－of－Recombinant－Proteins－in－SuspensionCultured－Plant－Cells
Plants have emerged in the past decade as a suitable alternative to the ?current production systems for recombinant pharmaceutical proteins and, ?toda
Preparation－of－human－platelets
Preparation of human platelets????? 1.?Human blood was taken from drug-free volunteers on the day of the experiment using acidic citrate dextrose
Steps－in－the－Glycosylation－of－Mammalian－Nlinked－Oligosaccarides
The biosynthesis pathway of N-glycans is a costly system with respect to the number of enzymes that are involved in the synthesis and trimming of N-gl
Human－Embryonic－Stem－(ES)－Cell－Protocols——Thawing－Human－ES－cells
Remove Human ES cells from liquid nitrogen storage tank. Fill out a freeze/thaw form.Thaw cryovial by gently swirling in waterbath until only a small
Human－Embryonic－Stem－(ES)－Cell－Protocols——Freezing－Human－ES－Cells
?Collagenase cells for approximately 7 minutes at 37 °C (until edges of colonies are curling up).With a 5 ml pipet, gently pipet and scrape colonies f
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Induction of BRF in bacteria and purification on Ni-NTA agaroseTransform BRF plasmid into strain BL21 DE3 (pLysS) or JM25 (DE3 containing the Arg tRNA
Characterization－and－Applications－of－PlantDerived－Recombinant－Antibodies
Expression of foreign proteins in plants has become a standard technique in plant molecular biology. Various plant species have been used to produ
重組質粒(dna－recombinant－plasmid)的連接
質粒具有穩定可靠和操作簡便的優點。如果要克隆較小的DNA 片段( ＜10kb) 且結構簡單，質粒要比其它任何載體都要好。在質粒載體上進行克隆，從原理上說是很簡單的，先用限制性內切酶切割質粒DNA 和目的DNA 片段，然后體外使兩者相連接，再用所得到重組質粒轉化細菌，即可完成。但在實際工作中，
Human－Embryonic－Stem－(ES)－Cell－Protocols—Splitting－Human－ES－cells－onMatrige
based on splitting onto on plateWarm collagenase media to 37°C in a water bath.Aspirate media off of cell culture plate.Add the following amount of co
Human－Embryonic－Stem－(ES)－Cell－Protocols——Splitting－Human－ES－cells－on－MEFs
based on splitting onto one plateWarm collagenase IV split media to 37 °C in a water bath.Aspirate media off of cell culture plate.Add the following a
典型CASE分享－蛋白產品常見翻譯后修飾（PTM）
翻譯后修飾（PTM）是指蛋白質在翻譯后發生的化學修飾。抗體在生產、貯存及臨床使用過程中，均可能產生各類翻譯后修飾變異體。翻譯后修飾可能導致抗體所帶的電荷乃至結構發生變化，從而影響其與抗原及Fc受體的親和力，進而影響抗體藥物的活性等關鍵質量屬性。因此，對抗體藥物的各類翻譯后修飾進行表征和工藝控制是有必
使用CO2恒溫搖床解決人胚腎－293－(HEK293)－細胞結團問題
人胚腎 293 (HEK293)? 細胞在重組蛋白表達中是最常見的宿主細胞。 這類細胞能夠表達大量的膜蛋白，如 G 蛋白偶聯受體? (GPCR) ，是無法在最常見的生物制藥生產宿主，如：中國倉鼠卵巢 (CHO) 細胞中作表達。 HEK293 雖然是蛋白表達的極好宿主，然而 HEK293 細胞