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Humanthyroidendothelialcellisolation

1. Human thyroid glands were obtained at surgery from multinodular goiters.2. The tissue was incubated in 0.1% type II collagenase (Worthington) overnight at room temperature.3. The digested tissue was passed through a 200-μm filter to remove undigested material, and the collagenase was removed by centrifugation at 360 g at 4°C for 10 min.4. The supernatant was discarded, and the cellular pell......閱讀全文