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1. Porcine eyes were harvested from pentobarbital-anesthetized animals, each weighing 30 to 40 pounds, and transported to the laboratory in ice-cold saline containing antibiotics.2. Each eye was hemisected with a circumferential cut posterior to the ora serrata, and the retinas were removed essentially intact after dissection at the optic nerve.3. Retinas were transferred to ice-cold primary ......閱讀全文
ContentsEmbryonic Stem Cell MarkersHematopoietic Stem Cell MarkersMesenchymal/Stromal Stem Cell MarkersNeural Stem Cell MarkersReferencesWhile stem ce
PREPARE SOLUTIONS1. 10mM MgSO4, 0.2% Maltose LB (100 mL):Mix 1.0 g of Bacto-Tryptone, 1.0 g of NaCl, 0.5 g of Yeast Extr
B. Midiprep double-stranded DNA isolationA midi-prep double-stranded DNA isolation has been developed to generate a sufficient amount of template DNA
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In VitroRoxanne Holmes and Juan Carlos Zú?iga-Pfl
實驗概要This protocol is intended for activation and expansion of human Treg cells isolated with the Dynal? CD4 CD25 Treg Kit (Cat. no. 113.23D). The exp
DNA Isolation by a Cleared Lysate Method Followed by Double Acetate Precipitation Version 3b - updated September 26, 1999The Most Recent Roe Lab Imple
Media and Solution required for ES Cell Culture (Bowtell Lab) Routine Culturing of ES Cells (Bowtell Lab) Routin
Isolation of retinal microvessels1. Eyes were obtained and transported on ice.2. Eyes were cut circumferentially 3 mm posterior to the limbu
實驗概要DNAzol? Reagent (Genomic DNA Isolation Reagent) is a complete and ready-to-use reagent for the isolation of genomic DNA from solid and
Coating of the Culture Dish SurfacesCulture dishes were incubated with a film of a 1:1 mixture of laminin (10 μg/ml and chondroitin sulfate (10 μg
實驗概要 This protocol utilizes the powerful guanidine isothiocyanate–phenol:chloroform extraction method which allows the
Isolation of AE Cells1. Human placentae were obtained with the approval of the institutional review board, after uncomplicated elective caesa
Isolation, Culture, and Differentiation of Progenitor Cells from the Central Nervous SystemScott R. Hutton and Larysa H. Pevny1UNC Neuroscie
MEDIA AND SOLUTIONS REQUIRED FOR ROUTINE ES CELL CULTURERoutine Culturing of ES CellsISOLATION OF PRIMARY MOUSE EMBRYO FIBROBLASTSMITOMYCIN C TREATMEN
Originally described in 1979 and more recently modified by Stamer et al primary trabecular monolayer cell culture has been a cornerstone for inves
實驗概要The E.Z.N.A.? family of products is an innovative system that radically simplifies extraction and purification of nucleic acids from a
Slide Coating13. Just before use, dilute the poly-D-lysine stock solution 1:50 in H2O. Add 0.2 mL of diluted poly-D-lysine to each well of a
Thyroid epithelial cells (also called follicular cells or principal cells) are cells of thyroid gland that are responsible for the production and
Isolation and Transcription Profiling of Purified Uncultured Human Stromal Stem CellsIsolation and Transcription Profiling of Purified Uncultured Huma
實驗概要E.Z.N.A.? Total RNA Midiprep Kit provides a rapid and easy method for the isolation of up to 600 ug of total RNA from cultured eukaryo
Introduction The most important aspect of our cloning vectors is that t
ADSC isolation, culture, and cloningRat amnion membrane is mechanically separated from the chorion of embryonic day 18.5 Sprague–Dawley rat embryosThe
實驗概要The E.Z.N.A.TM Fastfilter Plasmid Midi Kit combines the power of HiBind? technology with the time-tested consistency of alkaline-SDS lysis of
實驗概要The E.Z.N.A.TM Fastfilter Plasmid Midi Kit combines the power of HiBind? technology with the time-tested consistency of alkaline-SDS lysis of
Stromal stem cells proliferate in vitro and may be differentiated along several lineages. The scarcity of stromal stem cells in tissues and the la
Primary cultures of intrahepatic bile duct epithelial cells isolated and cultured1. Liver was surgically removed and perfused via the hepatic vei
實驗概要This protocols provides a general protocol for isolation of papillary cells.實驗步驟Isolation of renal papillary cells1. For isolation of p
可靠的CCCadvanced FN1無異源耗材支持人間充質干細胞擴增表達Reliable and Robust Animal-Component-Free hMSC-BM Expansion on Ready-to-Use Eppendorf CCCadvanced? FN1 Motifs Surf
實驗概要Neural stem cells (NSC) are valuable resources because of their ability to differentiate into neurons and glial cells with application
Isolation of limb muscle from neonatal mice1. Neonatal mice by decapitation or CO2 inhalation.2. Rinse the limbs with 70% ethanol and remo