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實驗概要This protocol is designed as a quick purification method for antibodies from mammalian sera, ascites, and cell culture supernatants. It should be noted that if the starting material is serum or ascites the final preparation will contain endogenous host IgG as well as specific antibodies. In general, the presence of this endogenous IgG should not interfere with assays using the antibodies. The immunogl......閱讀全文
實驗概要 The immunoprecipitation (IP) of cross-linked chromatin with antibodies specific for certain histone modifications (chromatin &nb
實驗概要ChIP is a powerful tool that allows the specific identification of proteins or histone modifications to regions of the genome. Chromat
Fine Mapping of Genomic Targets of Nuclear Proteins in Cultured CellsAchim Breiling and Valerio OrlandoDulbecco Telethon Institute, Institute of Genet
Metabolic biotinylation of mammalian cell receptors for imagingBakhos A. Tannous , btannous@hms.harvard.edu, Massachusetts General Hospital and Harvar
實驗概要We provide a general IP procedure including a list of reagents and a table to help you choose the correct protein beads.Immunoprecipit
借助Chromotek公司GFP-Trap如何設計成功的IP實驗？How to plan an immunoprecipitation of your GFP-fusion protein when using the ChromoTek GFP-Trap?PreambleThis document
E. Elution of DNA fragments from agaroseDNA fragments are eluted from low-melting temperature agarose gels using an unpublished procedure first develo