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實驗概要We provide a protocol for fixation, immunostaining, and imaging in 384-well Plates.主要試劑Reagents1. 384-well view plates (Aurora)2. HUVEC (pooled, Lonza)3. EBM-2 Medium (Lonza)4. Fixation Solution - 16% Paraformaldehyde (PFA) aqueous solution (diluted to 4% in wells)5. 1x PBS w/o Ca2 and Mg2 (prepared from powder)6. Donor Calf Serum (DCS)7. Triton? X-1008. Primary antibody ......閱讀全文
Immunofluorescence Technique (Spector Lab)protocol for immunofluorescence on cells Immunofluorescence Protocol (Walter Steffen)Me
Genome-wide Gene Expression Analysis (Richard Young Research Group,Whitehead Institute for Biomedical Research)A genoe-wide gene expression analy