實驗概要Sandwich enzyme-linked immunosorbent assays (ELISAs) involve attachment of a capture antibody to a solid phase support. Samples containing known or unknown antigen are then added in a matrix or buffer that will minimize attachment to the solid phase. An enzyme-labeled antibody is then added for detection.The ELISA method is a benchmark for quantitation of pathological antigens ......閱讀全文
實驗概要This protocol provides a general protocol for human B cell isolation and culture.實驗步驟B cell isolation1. Donor blood was obtained with informe
General Protocol for the Sandwich ELISA method:Before the assay, both antibody preparations should be purified and one must be labeled.For most a