WIKI資訊
1) The yeast species Saccharomyces cerevisiae were cultured in SD media with 2% raffinose. Before extraction, yeast were precultured, diluted to 0.2 OD600 units/ml and cultured to 2 OD units/ml, washed and stored at -80oC. 2) Yeast cells were resuspended in 150 mM NH4HCO3 (pH 8) and disrupted by zirconia beads (0.5 mm; BioSpec Products). 3) Yeast ......閱讀全文
實驗概要The Dynabeads? and buffers provided in this kit will enable you to a) covalently immobilize antibodies of your choice onto the surface
My Experience Purifying RNA from E. coliRegarding RNA extraction, there is a horrible tendency of people to use kits for RNA extraction with bacteria.
實驗概要The Dynabeads? and buffers provided in this kit will enable you to a) covalently immobilize antibodies of your choice onto the surface
實驗概要Plant DNAzol? is an extra-strength-DNAzol? reagent (patent pending) specifically formulated for the isolation of genomic DNA from plants. The Plan
IntroductionFluorescence in situ hybridization (FISH) has become a widely used method in genome and molecular genetic studies. The technique
Methylated CpG island Amplification Protocol written by Minoru Toyota2. Materials2.1. MCARestriction enzymes SmaI, XmaIT4 DNA ligaseTaq DNA polym
1) Immunocapture stageCoating buffer: 15 mM Na2CO3; 35 mM NaHCO3, and 3 mM NaN3, per liter (pH 9.6).Extraction buffer: (20 mM Tris-HCL (pH 8.0),
1) Immunocapture stageCoating buffer: 15 mM Na2CO3; 35 mM NaHCO3, and 3 mM NaN3, per liter (pH 9.6).Extraction buffer: (20 mM Tris-HCL (pH 8.0),
Modified from that of Jay Thelen - University of Missouri-ColumbiaPhenol extraction followed by methanolic ammonium acetate precipitation - an effecti
1. Name：Tuning structure and properties of ultra-low cross-linked temperature-sensitive microgels at interfaces via the adsorpti
實驗概要This procedure does not require phenol extraction. The DNA is pure enough for restriction digests, PCR and genomic library construction.High t
3. INTERFEROMETER PERFORMANCEFigure 3 above shows both interferometer outputs verses time, along with plasma discharge current on the LArge
Preparation of fungi for DNA extraction typically involves growing cultures in liquid culture in Erlenmeyer flasks, Roux bottles or even microfuge tub
BCEIA2019 International Summit on Scientific Instruments Development-- Registration in progress China is one of the world’s fastest growing analytical
實驗概要DNA extraction from tissue.主要試劑Extraction buffer100 mM Tris-HCl (pH 8.0) 100 mM EDTA (pH 8.0) 100 mM Na-Phosphat
實驗概要The procedure presented below describes a facile method for studying signal transduction events with suspension cells (Jurkat, Raji, T
實驗概要The procedure presented below describes a facile method for studying signal transduction events with suspension cells (Jurkat, Raji, T
實驗概要The procedure presented below describes a facile method for studying signal transduction events with adherent cells (HeLa, MCF-7, BALB
OverviewHigh throughput of many fungal isolates can be achieved by growing axenic cultures in either (a) 1.5mL microfuge tubes, half full with liquid
實驗概要The procedure presented below describes a facile method for studying signal transduction events with adherent cells (HeLa, MCF-7, BALB
OverviewHigh throughput of many fungal isolates can be achieved by growing axenic cultures in either (a) 1.5mL microfuge tubes, half full with liquid
The available procedures for isolation and purification of photosystem I (PSI) from Chlamydomonas reinhardtii are time consuming and usually require
Removal of Ethidium Bromide from DNA by Extraction with Organic SolventsJoseph SambrookPeter Maccallum Cancer Institute and The University of Melbourn
General InformationPhenol/chloroform extraction is an easy way to remove proteins from your nucleic acid samples and can be carried out in a manner th
BCEIA2019 International Summit on Scientific Instruments Development--Registration in progressChina is one of the world’s fastest growing analytical a
RNA extraction with TRIzol (Invitrogen product name) or the equivalent TRI (Sigma-Aldrich product name) is a common method of total RNA extractio
We have found that the DNA extraction procedure of Metzenberg and Baitch (Neurospora Newsl. 28:20)/Stevens and Metzenberg (Neurospora Newsl. 29:27) wh
實驗概要The procedure presented below describes a facile method for studying signal transduction events with suspension cells (Jurkat, Raji, T
Differential cDNA Screening ProceduresThe protocols listed refer to cDNA library construction and preliminary differential screening procedures. They
實驗概要The E.Z.N.A.? Total RNA Maxi Kit uses HiBind? matrix spin-column technology to isolate up to 5 mg total cellular RNA from a variety of