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Terminal differentiation of cells is often accompanied by repression of cellular proliferation, suggesting that there is a mechanism by which these cellular functions are coordinated. Macrophage differentiation is one model system in which this occurs; as macrophages differentiate, they also stop proliferating. Transcriptional regulation plays a key role in cell cycle progression as well as many differentiation proce......閱讀全文
TROUBLESHOOTINGProblem: The OP9 cells are more than 80%-90% confluent.Solution: It is important when creating working stocks of OP9 cel
EPC Isolation and Characterization1. EPCs were obtained by isolating mononuclear cells using Ficoll density-gradient centrifugation of human bloo
Protocol 3: Fetal Liver-Derived HSC Differentiation on OP9-DL1 CellsDay 0: Initiation of Fetal Liver Co-culture52. Isolate liver tissue from eight to
In this study, we found that “acute” response was strongly induced by cryo-thermal therapy with “acute” and high expression of a series of acute p
ObjectiveThe objective of the experiment is to determine the effects of ethanol exposure on the embryonic development of zebrafish through observation
在免疫學實驗中，比如WB，IHC，IF等，大家都知道選著好正確的一抗，往往實驗就成功了一大半，因此，往往很多研究者便會忽視對二抗，以及其它試劑盒品質的追求。二抗是一種相對而言比較穩定的產品，包裝大，因此很多實驗室都會公用一個二抗。但如果二抗沒有選擇好的話，影響的可能就是一整個實驗室或者課題組了。艾美
FluidFM 測定細胞粘附力的應用隨著時間推移，越來越多的學者開始使用FluidFM 技術進行測定細胞粘附力。以下就近五年的具有代表性的應用進行總結。Cohen 等使用FluidFM 技術對MCF7-MCF10A、MCF7-HS5 的細胞粘附力進行了測定，并與以往的文獻進行對比，發現其數據與Hos
四、展望從1991 年Fodor 提出芯片這個概念到現在,已有2 000 多篇各類與芯片有關的文章發表,其中絕大多數是在1999 年以后發表的。這說明基因芯片在最近二三年發展迅速,應用廣泛。目前,對于基因芯片的研究朝著快速、高質量、準確的方向高速發展。雖然基因芯片仍有許多問題有待解決,但其前景一片光
IntroductionFetal Alcohol Syndrome (FAS) is caused by exposure of the developing embryo to alcohol, one of several teratogenic agents which adversely
美國BARTZ公司最早研制生產微根窗根系觀測儀器，BTC-100成為國際上應用最廣、發表論文最多的根系研究儀器技術，其學術研究論文涵蓋幾十種世界知名學術期刊和專著，包括Science、Nature、Global change biology（影響因子8.502）、New
實驗概要The protocols in this section describe the steps involved in differentiating neural stem cells (NSC) to neurons, astrocytes, and oligo
Low-density lipoprotein (LDL) is a plasma lipoprotein particle whose lipid component includes cholesterol and triglycerides and is commonly referred t
Macrophages and NK cells help provide innate immunity against infection by intracellular parasites and communicate with each other to regulate this pr
2011CJ Bernacchi, ADB Leakey, BA Kimball, DR Ort (2011) Growth of soybean at future tropospheric ozone concentrations decreases canopy evapotransp
Primary referencestop American Journal of Clinical Pathology (AJCP), August 1975 to February 2006American Journal of Surgical Pathology
實驗概要Glial precursor cells (GPCs), also known as glial restricted progenitors (GRP) or oligodendrocyte progenitor cells (OPCs), are cells t
While T cells and B cells carry out the actions of antigen-specific immune responses, antigen-presenting cells called dendritic cells are required for
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In VitroRoxanne Holmes and Juan Carlos Zú?iga-Pfl
Objective: This experiment aims to discover if temperature has an effect on the rates of sea urchin embryonic development. By altering the extern
諾華（Novartis）制藥 Wang, Karen（王瑛琪）教授 來自諾華（Novartis）制藥的Wang, Karen（王瑛琪）教授，做了題為“Application of Mass Spectrometry in Pharmaceutical Drug Discovery Research
For ionisation to take place at all, chemical reaction between the sample and the&nbs
ObjectiveThis experiment attempted to determine the effects of the teratogen cyclopamine on the Shh signaling pathway in axolotl embryos. Groups of ax
Flow cell assays are very useful for studying microtubule motility, microtubule dynamics, kinetochore-microtubule interactions and action of severing/
實驗概要Dopaminergic (DA) neurons are located in the ventral midbrain (VM). The ability to isolate precursor cells and neurons from the VM pro
More than 22 million American women smoke and approximately 25% of these women continue to smoke during pregnancy (Lambers and Clark 1996). Cigarette
I. INTRODUCTION 引言 This guidance helps sponsors of investigational new drug applications (INDs) or applicants of new drug applications (NDAs),
實驗概要Directed differentiation of specific lineages has been a focal point in the field of human embryonic stem cell (hESC) research. Cell r
ContentsEmbryonic Stem Cell MarkersHematopoietic Stem Cell MarkersMesenchymal/Stromal Stem Cell MarkersNeural Stem Cell MarkersReferencesWhile stem ce
Objective The purpose of this experiment is to observe the effect of caffeine on the heart rate of three day old ch