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實驗概要Invitrogen offers several fluorescent and biotinylated phalloidin and phallacidin derivatives for labeling F-actin. These phallotoxins, isolated from the deadly Amanita phalloides mushroom, are bicyclic peptides that differ by two amino acid residues. They can be used interchangeably in most applications and bind competitively to the same sites in F-actin. Phalloidin and phalla......閱讀全文
AcO- --- part one ---(AcO)2Ca 1; 1.5MAcOK 1; 2; 3; 4; 5; 6; 7; 8M(AcO)2Mg 1M
Vasoactive intestinal peptide (VIP) and the structurally related pituitary adenylate cyclase-activating polypeptide (PACAP), two neuropeptides present
Organic substances.pKa and temperature dependence of pH for common buffers.ATP 0.1MBetaine 5MCresol red (Na) 50mMDTT 1M, 2.2M
CONTENTPCR guide: a discussion of the main parameters influencing the outcome of the PCR and multiplex PCR reaction in 16 pages/sections and using ove
F. Video Microscopy 1. The behavior of migrating cells may be filmed with an inverted microscope fitted with a CCD camera. 2. Determine the
實驗概要The iPrep? GeneCatcher? gDNA Blood Kit allows rapid and automated extraction of genomic DNA (gDNA) from human blood including archived
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In VitroRoxanne Holmes and Juan Carlos Zú?iga-Pfl
Mammalian RNA InterferenceThomas TuschlLaboratory for RNA Molecular BiologyThe Rockefeller University, New York Excerpted from RNAi: A Guide
The most recognized mechanism by which peroxisome proliferators regulated gene expresssion is through a PPAR/RXR heterodimeric complex binding to a pe
In Situ Hybridization· In Situ Hybridization (jsmith1@po-box.mcgill.ca)In situ hybridization
if mouse is found deadif mouse is a newborn or < 1 wk of ageIf mouse is alive or moribund and above one week of ageRoutine dissectionFormalin fixat
Protocols for LCM preparation and analysis I. Preparation, LCM and RNA/DNA extraction of Frozen Tissue SectionsA. EmbeddingB. CuttingC.
Immunofluorescence Technique (Spector Lab)protocol for immunofluorescence on cells Immunofluorescence Protocol (Walter Steffen)Me
最方便的實驗干貨查詢工具微信掃碼進入「丁香實驗」小程序編輯： 嗚咽分享到： Flow Cytometry Analysis (Springer Lab, Harvard University)Flo
Flow Cytometry Analysis (Springer Lab, Harvard University) Flow cytometry employs instrumentation that scans single cells flowing past excit
Western 雜交（主要內容如下）Preparing of Protein LysatesWestern BlottingFar Western BlottingSemi Dry BlottingStripping MembranesTrouble Shooting and Others
蛋白質電泳（主要內容如下）One-Dimensional SDS-PAGETwo-Demensional SDS-PAGEProtein Electrophoresis in Agarose Gel Gel StainingRecipesOne-Dimensional SDS-PAGE·&
實驗概要During more than 25 years of application in immunological sciences, ELISPOT has been established as a routine, robust, versatile, and
實驗概要本實驗介紹了動物細胞基因組DNA中小衛星多態性Southern blot檢測的原理和操作步驟。掌握核酸雜交檢測技術（Southern blotting技術）、核酸探針的標記技術、小衛星DNA多態性檢測分析技術（DNA 指紋圖譜技術）和化學發光檢測技術。實驗原理Southern印跡雜交技術包括
DNA抽提（主要內容如下）· Working with DNA· DNA Extraction from Bacteria and Other Organisms· DNA Extraction f
GenJet Ver.II，LipoD293及PolyJet針對哺乳動物細胞的DNA體外轉染試劑，均為您提供兩種不同的轉染步驟——一般步驟及高級步驟，這兩種步驟分別針對不同的哺乳動物細胞。高級步驟主要針對難轉的哺乳動物細胞，例如：MDCK，MDA-MB231，Caco-2等細胞。使用一般的轉染步
Andrea J. Gossett and Jason D. Lieb1Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA1Correspon
DNA轉化Chemical Transformation· Transformation of Competent Cells (RbCl2 Method) (Goldberg Lab
Manipulating and analyzing DNA are fundamentals in the field of molecular biology. Indeed, separating complex mixtures of DNA into different sized fra
· Double Peroxidase (HRP) Immunohistochemical Labeling of Trypsin-Sensitive Antigens (KPL)·
IntroductionRNAse A treatment of permeabilized cells followed by immunostaining is a method which allows to show if the localization of a protein into
This page should point you to the many different general and lab-specific protocols describing tissue and cell lysis and serve as forum for comparison
· Standard (alkaline lysis) Mini-Prep (Goldberg Lab)Standard protocol for mini-prep and reci
· Standard (alkaline lysis) Mini-Prep (Goldberg Lab)Standard protocol for mini-prep and reci
Electrophoresis through agarose or polyacrylamide gels is the standard way to separate, identify and purify nucleic acid fragments. The location of th