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Andrea J. Gossett and Jason D. Lieb1Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA1Corresponding author (jlieb@bio.unc.edu)INTRODUCTIONKnowledge of the DNA-binding specificity of a transcription factor aids in understanding the function of that factor in the regulation of gene transcription. One popular method ofidentifying the genomic DNA sites boun......閱讀全文
Table 2: Binding sites and identity of ZFPs used in VEGF activationWe then generated artificial transcription factors by fusing the three-fi
Or gel mobility shift assay, gel shift assay, gel retardation, electrophoretic mobility shift assay (EMSA) EMSA Using Oligos (Mike A. Dyer)A
Human zinc fingers as building blocks in the construction of artificial transcription factorsKwang-Hee Bae1, 4, Young Do Kwon1, 2, 4, Hyun-Chul Shin1,
Scanning the human genome with combinatorial transcription factor librariesPublished online: 18 February 2003, doi:10.1038/nbt794March 2003 Volume 21
The International Working Group on Antibody Validation: 國際抗體驗證工作組 Proposal for Antibody Specificity Testing Q&A 關于抗體特異性檢測提議的常見問題及回答 1.What i
1. Introduction and BackgroundThere is a great need for general methods to characterize the proteins that contemporary biology makes available. The li
Figure 5: Regulation of CDH5 by TFZFs in several human cancer cell lines.Blue, cells infected with a pMX construct containing the DNA bindin
AbstractChIP-Chip stands for Chromatin Immunoprecipitation and chip in the sense of DNA microarray. It is a technique to determine the genom
實驗概要 The immunoprecipitation (IP) of cross-linked chromatin with antibodies specific for certain histone modifications (chromatin &nb
2.2 Interaction mating - large scaleWith a few modifications, the procedure described above can be used to test for interactions between a single prey
Methylation Specific PCRProtocol written by James Herman*Methylation Specific PCR (MSP) is a simple rapid and inexpensive method to determine the meth
ConclusionsQuorum-sensing signaling systems involving the interaction between a signaling peptide and its cognate histidine kinase receptor are widely
ProtocolsDownloadprotocol183kbpdf?Abstract for Gel Shift Assay SystemsThe gel shift, or electrophoretic mobility shift, assay provides a simple a
ANALYSIS OF CELL CYCLE Miriam Capri and Daniela Barbieri Dept. Biomedical Sciences, Sect. General Pathology,Via Campi, 287, University of Mo
借助Chromotek公司GFP-Trap如何設計成功的IP實驗？How to plan an immunoprecipitation of your GFP-fusion protein when using the ChromoTek GFP-Trap?PreambleThis document
Cellular Transfection and Immunoprecipitation Before proceeding with the experiments outlined below, all kinase pocket mutants should be ch
ASTRACTWe describe a rapid and quantitative flow cytometric method for determining the apoptotic or anti-apoptotic potential of a gene in various cell
Footprinting Procedures· DNase I Footprinting (Mike A. Dyer)· &
實驗概要The exponential amplification via reverse transcription polymerase chain reaction provides for a highly sensitive technique in which a
INTRODUCTION After chromatin immunoprecipitation (ChIP), different PCR-based approaches can be used to determine how much DNA is p
ContentsFactors Affecting the PCR Nested Primer PCRPrimer LengthDegenerate PrimersElongation Temperature and TimeReaction BufferCycle Numbe
Fine Mapping of Genomic Targets of Nuclear Proteins in Cultured CellsAchim Breiling and Valerio OrlandoDulbecco Telethon Institute, Institute of Genet
INTRODUCTIONAfter chromatin immunoprecipitation (ChIP), different PCR-based approaches can be used to determine how much DNA is precipitated at a locu
METHOD Analysis of precipitated chromatin fractions (from Chromatin Immunoprecipitation on Unfixed Chromatin from Cells and Tissues to Analy
General AdvicePCR allows the production of more than 10 million copies of a target DNA sequence from only a few molecules. The sensitivity of this tec
INTRODUCTIONAfter chromatin immunoprecipitation (ChIP), different PCR-based approaches can be used to determine how much DNA is precipitated at a locu
The restriction/modification system in bacteria is a small-scale immune systemfor protection from infection by foreign DNA. W. Arber and S.
分析測試百科網訊 2015年9月9日-12日，第五屆金屬組學國際研討會在北京西郊賓館召開，會議由中國科學院科院高能物理研究所、清華大學共同主辦，來自世界各地的近200位金屬組學領域的專家學者匯聚一堂，探討金屬組學的最新進展及未來展望。12日的分會場一，來自復旦大學的Xiangshi T
DNA and RNA Staining6. Stain cells with 7-AAD: i. Resuspend the cells from Step 5 in 0.5 mL of NASS containing 10 μg/mL of 7-AAD. Incub
Application: Quantitative RT-PCR is used to quantify mRNA in both relative and absolute terms. It can be applied for the quantification of m