AbC?AntiMouseBeadKit
實驗概要The AbC? Anti-Mouse Bead Kit provides a consistent, accurate, and simple-to-use technique for the setting of flow cytometry compensation when using fluorochrome-conjugated mouse antibodies (Picture 1). The kit contains two types of specially modified polystyrene microspheres, the AbC? capture beads, that bind all isotypes of mouse immunoglobulin, and the negative beads that hav......閱讀全文
AbC?－AntiMouse－Bead－Kit
實驗概要The ?AbC? Anti-Mouse Bead Kit provides a consistent, accurate, and ?simple-to-use technique for the setting of flow cytometry compensation ?when u
AbC?－AntiMouse－Bead－Kit
實驗概要The ?AbC? Anti-Mouse Bead Kit provides a consistent, accurate, and ?simple-to-use technique for the setting of flow cytometry compensation ?when u
Th17－Polarization－of－Mouse－CD4－Cells
實驗概要T ?helper 17 (Th17) cells are a subset of CD4 T helper cells characterized ?by their production of IL-17, particularly IL-17A and IL-17F. They are
Th9－Polarization－of－Mouse－Splenocytes
實驗概要Th9 ?cells are a subpopulation of T-helper cells, characterized to be ?involved in allergic diseases and resistance against intestinal ?nematodes.
Immunofluorescent－staining－of－Sea－Urchin－embryos
1. Transfer fixed embryos to microfuge tubes. Allow to settle for 10 minutes.Gently remove most of the liquid.2. Add 100 ul antibody to one tube and 1
ImmunohistochemistyFluorescence－Protocol1
MaterialsCytokine-specific Primary Antibodiesunlabeled or biotinylated antigen-affinity purified polyclonal antibodies (R&D Systems ''AF'&
3－Color－Staining:－AlphaCatenin－in－HeLa－Human－Cervical－Cancer－Cells
實驗概要Alpha-catenin ?in HeLa human cervical cancer cells was labeled using mouse ?anti-α-catenin and visualized with Alexa Fluor? 488 goat anti-mouse Ig
二抗的選擇和定位
第一步：選擇抗體。三種二抗抗體：Whole IgG，F(ab&；rsquo；)2片段，Fab片段。?Whole IgG抗體適用于多數情況，是性價比最高的。Whole IgG是完整的抗體分子，有一個Fc部分和兩個與抗原結合的Fab部分 (Figure 1, Jackson ImmunoResearch
Immunodetection－of－cyclin－D1－and－D2/D3－using－flow－cytometry
DescriptionThis protocol is for use with the D cyclins and employs 488 nm argon laser excitation of propidium iodide and 630 nm NeNe or diode laser ex
二抗的選擇
二抗應選用與使用的一抗相同的物種來源，例如：如果你的一抗是小鼠的單克隆抗體，二抗則選抗小鼠的二抗anti-mouse?secondary。建議檢查二抗說明書確保該抗體適用于你的檢測應用，?二抗一般連接熒光素FITC或發光團。
小鼠Treg檢測操作步驟
1、在每個流式上樣管中加入100?μl準備好的細胞懸液，細胞數約為1x106個.2、按照細胞表面抗原染色方法標記表面抗原。根據說明書加入CD4和CD25抗體100?μl體系中使用0.125?μg?FITC?anti-mouse?CD4，0.06?μg?APC?anti-mouse?CD25抗體。最好
ALKALINE－PHOSPHATASE－(APAAP)－TECHNIQUE
Preparation: Cytological Preparations Fixation: Air dry films or cytospin preparations overnight at room temperature. For frozen and paraffin secti
Early－development－of－primary－motor－neurons－and－somites－in－Zebrafish－Embryos
Background:Zebrafish,or the teleost fish Danio rerio,is a rapidly developing organism that is apopular species for studying vertebrate development. Cl
Cell－Surface－Immunofluorescence－Staining－Protocol
實驗概要A method of identifying ?and enumerating specific cell types in a heterogeneous population of ?cells by enhancing the specific staining of desired
間標磁珠——輕松分選任意細胞
我們知道，做細胞分選，首先要知道目的細胞與其它細胞相比，有什么特別的標志，如常用的細胞表面CD marker，最簡單的方法是選擇直接耦聯相應抗體的磁珠，即所謂的直標微珠，如用CD4 microbeads來直接分選CD4 + T細胞。 但是，美天旎的直標磁珠多是針對小鼠、大鼠、人和靈長類的，針對其它物
Brdu免疫組織化學染色分析－BrdU－incorporation－assay
Enzyme Immunostaining for BrdU:Wash frozen sections with PBS 2x.Put them in 0.1% Pepsin in 0.1N HCL (in PBS) at 37?C for 50min.Then in 0.3% hydrogen p
LIVE/DEAD?－Fixable－Dead－Cell－Stain－Kits
實驗概要The ?LIVE/DEAD? Fixable Dead Cell Stain Kits use a novel method to evaluate ?the viability of mammalian cells by flow cytometry. These assays are
specific－immunodetection－of－cyclins－using－488/630－dual－laser－flow－cytometry
Phenotype-specific immunodetection of cyclins using?488/630 nm dual laser flow cytometry William Telford? Hospital for Special Surgery This pr
Indirect－ELISA
實驗概要?In ?the indirect ELISA, the enzyme-antibody conjugate uses an antibody ?against the type of antibody that is used to detect the antigen, kind of
Immunohistochemistry－using－AntiGanglioside－Antibodies
Immunohistochemistry using Anti-Ganglioside Antibodies Tadashi Tai~Head, Department of Tumor Immunology, The Tokyo Metropolitan Institute of Medi
LIVE/DEAD?－Fixable－Dead－Cell－Stain－Kits
實驗概要The LIVE/DEAD? ?Fixable Dead Cell Stain Kits use a novel method to evaluate the ?viability of mammalian cells by flow cytometry. These assays are
Largescale－Immunocytology
This protocol describes our method for preparing cells for immunofluorescence, in which all incubations and washes are performed in microtiter dishes
Wholemount－staining－of－embryos
Fix embryos in formalin or MEMFA for one hour at room temperature with mixing. Rinse with TBS, replace with methanol, store at -20oC. Rehyd
ELISA－with－Platelet
OUTLINE This modification of qualitative ELISA (Enzyme-Linked Immunosorbent Assay) is used for either screening detection of anti-platelet antibodie
單抗的研究進展
單克隆抗體藥物的發展起源于1975年,雜交瘤技術的問世使大量制備均一的鼠源單克隆抗體成為可能。1986年第一個抗移植后免疫排斥反應的鼠源單克隆抗體muromonab-CD3(OKT3)經美國食品藥品監督管理局（Food and Drug Administration, FDA）批準上市， 但是來
單克隆抗體的研究進展
單克隆抗體藥物的發展起源于1975年，雜交瘤技術的問世使大量制備均一的鼠源單克隆抗體成為可能。1986年第一個抗移植后免疫排斥反應的鼠源單克隆抗體muromonab-CD3(OKT3)，經美國食品藥品監督管理局（Food and Drug Administration, FDA）批準上市，[2]
關于單克隆抗體的研究進展介紹
單克隆抗體藥物的發展起源于1975年，雜交瘤技術的問世使大量制備均一的鼠源單克隆抗體成為可能。1986年第一個抗移植后免疫排斥反應的鼠源單克隆抗體muromonab-CD3(OKT3)，經美國食品藥品監督管理局（Food and Drug Administration, FDA）批準上市，但是來
單克隆抗體藥物的發展起源
單克隆抗體藥物的發展起源于1975年，雜交瘤技術的問世使大量制備均一的鼠源單克隆抗體成為可能。1986年第一個抗移植后免疫排斥反應的鼠源單克隆抗體muromonab-CD3(OKT3)，經美國食品藥品監督管理局（Food and Drug Administration, FDA）批準上市，但是來源于
單克隆抗體的研究進展
單克隆抗體藥物的發展起源于1975年，雜交瘤技術的問世使大量制備均一的鼠源單克隆抗體成為可能。1986年第一個抗移植后免疫排斥反應的鼠源單克隆抗體muromonab-CD3(OKT3)，經美國食品藥品監督管理局（Food and Drug Administration, FDA）批準上市，[2]
Rodent－Retinal－Ganglion－Cell－Cultures
實驗概要Central neurons lose the ability for axonal regrowth during development and typically do not regenerate their axons following axotomy once the