ImmunohistochemistryusingAntiGangliosideAntibodies
Immunohistochemistry using Anti-Ganglioside AntibodiesTadashi Tai~Head, Department of Tumor Immunology, The Tokyo Metropolitan Institute of Medical Science, Tokyo, JapanAn indirect method using immuno- fluorescence coupled to second antibody is a relatively simple, rapid, and easy immuno-histochemical procedure. It is best suited for the study of membrane antigens in addition to intra- and extracellular antigens, and......閱讀全文
Immunohistochemistry－using－AntiGanglioside－Antibodies
Immunohistochemistry using Anti-Ganglioside Antibodies Tadashi Tai~Head, Department of Tumor Immunology, The Tokyo Metropolitan Institute of Medi
Conjugation－of－monoclonal－antibodies
Conjugation of monoclonal antibodiesPrelude: You are free to copy and distribute these documents at will--but please do so in their entirety, complete
Tips－and－hints－for－the－storage－of－antibodies
Storage of lyophilized antibodiesAll our products are shipped lyophilized (freeze-dried). Unlabeled antibodies are stable without loss of quality at a
Protein－G－Purification－of－Antibodies
1. Reagent and Materials(1) Hi-Trap Protein G Column (Pharmacia Biotech #17-0404-01)(2) 20 mM Sodium Phosphate Buffer, pH 7.01.084 g NaH2PO4, anhydrou
Coupling－Antibodies－to－Protein－A－or－G
1. use 2 mg of antibody per ml wet beads (use appropriate antibody/protein A or G combination).2. mix antibodies with beads and bind at room temperatu
Antigen－Affinity－Purification－of－Antibodies
實驗概要To acquire purified antibodies (This method typically yields >95% pure specific antibodies ).實驗原理?Cytokines ?are signaling proteins necessary for
Using－GenBank
GenBank(R) is a comprehensive database of publicly available DNA sequences for more than 205,000 named organisms and for more than 60,000 within t
Using－a－Counting－Chamber
Using a Counting ChamberFor microbiology, cell culture, and many applications that require use of suspensions of cells it is necessary to determine ce
SingleChain－Fv－Antibodies－Expressed－in－Plants
Methods of gene cloning and genetic engineering of immunoglobulins and transgenic plant techniques have given rise to new approaches in plant bio
Characterization－and－Applications－of－PlantDerived－Recombinant－Antibodies
Expression of foreign proteins in plants has become a standard technique in plant molecular biology. Various plant species have been used to prod
Deglycosylation－of－Glycoproteins－Using－Endoglycosidases
T.H. Plummer, Jr. and A.L. Tarentino, Department of Biochemistry, New York State Department of Health, Wadsworth Center for Laboratories and Research
ChIP－using－plant－samples
實驗概要?The ?immunoprecipitation (IP) of cross-linked chromatin with antibodies ?specific for certain histone modifications (chromatin ?immunoprecipitati
Method:－Cell－Counts－Using－a－Hemacytometer
Method: Cell Counts Using a Hemacytometer June 1, 1990 Rosalie Veile Purpose: The purpose of this procedure is to determine the cell d
Transfection－of－Mammalian－Cells－Using－Lipofectamine
Materials:??????? Lipofectamine Basal Medium containing 10% fetal bovine serum, 1% glutamine, 1% aa Basal Medium containing 1% glutamine
Extraction－of－DNA－using－DNAzol?－Reagent
實驗概要DNAzol? ?Reagent (Genomic DNA Isolation Reagent) is a complete and ready-to-use ?reagent for the isolation of genomic DNA from solid and liquid sa
RNA－extraction－using－trizol/tri
RNA extraction with TRIzol (Invitrogen product name) or the equivalent TRI (Sigma-Aldrich product name) is a common method of total?RNA extraction?fro
Using－GenBank－for－Genomic－Authentication:－A－Tutorial
The GenBank? database is perhaps one of the most important repositories of genetic information. A researcher working in the field of genomic authe
ChIP－using－plant－samples－–－Arabidopsis
實驗概要This protocol describes how chromatin is prepared from Arabidopsis, which can subsequently be used for chromatin immunoprecipitation (ChIP). T
Fluorescence－In－Situ－Hybridization－using－TSA?
實驗概要This ?protocol describes steps for fluorescent in situ hybridization (FISH) ?to Drosophila embryos using Tyramide Signal Amplification (TSA?), and
FACS－Analysis－Using－Peripheral－Blood－Cells
FACS Analysis Using Peripheral Blood Cells Collect blood (75 microliters) into 1ml PBS containing 5 microM EDTA (10 microliters of 0.5 M stock) a
Assay－of－Tyrosine－Kinases－Using－Synthetic－Peptides
實驗概要 ? ? ? ? Small synthetic peptide substrates are especially well suited for applications such as assays of tyrosine kinases in permeabilized
Cloning－PCR－products－using－TA－vectors
Cloning PCR products using TA vectorsby Paul N. Hengen, Ph.D.?*Methods and reagents is a unique monthly column that highlights current discussions in
Sitedirected－Mutagenesis－using－PCR
Site-directed Mutagenesis using PCRMichael P. Weiner, Tim Gackstetter, Gina L. Costa, John C. Bauer, and Keith A. KretzFrom:?Molecular Biology: Curren
Detection－of－apoptotic－process－in－situ－using－immunocytochemical
1. INTRODUCTION??Apoptosis was observed from invertebrates to lower and higher verterbrates, and intervenes both in physiological and in pathological
Yeast－Gene－knockout－using－Oligo/PCR
Universal primers for gene knock-out using dominant drug markers: Kan, Clonat, and Hygromisin-B.?Forward primer: 5’ TCAGGGGCATGATGTGACT 3’Reverse prim
一種基于DNA的通用型蛋白檢測系統
摘要：基于抗體的蛋白質檢測方法，主要有western blot、ELISA、點雜交以及免疫組化等，這些方法被廣泛地應用于科研和診斷領域。在蛋白質的免疫檢測過程中，樣品蛋白首先結合與特異性一抗上，然后再用攜帶標簽（諸如：熒光染料，放射性元素，酶等）的二抗進行檢測。然而，為了避免種間內的交叉反應，必
Studying－Arabidopsis－Envelope－Protein－Localization－and－Topology－Using－...
Chloroplasts are metabolically important organelles that perform many essential functions within plant cells. The chloroplasts can be subdivided
Purification－of－Genomic－DNA－Using－PureLink?－Silica－Columns
實驗概要 The PureLink? ?Genomic DNA Purification Kit allows rapid and efficient purification of ?genomic DNA. The kit is designed to efficiently isolate
Expression－L19－using－Pichia－pastoris
Pichia pastoris is a methylotropic yeast used to express high amounts of protein. Secreted expression is achieved with a cleavalable faktor. To yield?
Detection－of－MicroRNA－Heterogeneity－in－Single－Cells－Using－an－Automated
Introduction ?MicroRNA ?(miRNAs) are short (18–24 nucleotides), non-coding RNAs that regulate ?gene expression by both disrupting messenger RNA (mRNA