WIKI資訊
ProcedureI. INTRODUCTIONThe 293 EBNA cell line is established from primary embryonal human kidney cells transformed with sheared human adenovirus type 5 DNA and Epstein-Barr virus nuclear antigen 1, allowing episomal amplification of plasmids containing the viral EBV origin of replication. This protocol describes the instructions for the growth, maintenance and transfection of suspension adapted 293-EBNA cell line us......閱讀全文
人胚腎 293 (HEK293) 細胞在重組蛋白表達中是最常見的宿主細胞。 這類細胞能夠表達大量的膜蛋白，如 G 蛋白偶聯受體 (GPCR) ，是無法在最常見的生物制藥生產宿主，如：中國倉鼠卵巢 (CHO) 細胞中作表達。 HEK293 雖然是蛋白表達的極好宿主，然而 H
實驗概要293fectin? is a proprietary, cationic lipid-based formulation for transfection of DNA into eukaryotic cells. 293fectin? is optimized f
Mammalian RNA InterferenceThomas TuschlLaboratory for RNA Molecular BiologyThe Rockefeller University, New York Excerpted from RNAi: A Guide
Lysate preparation and western blottingProtein lysates were created by harvesting the cells from con?uent T-?asks or from suspension cultures at h
Lentivirus Transduction of Hematopoietic CellsMing-Jie Li and John J. RossiDivision of Molecular Biology, Beckman Research Institute of the City of Ho
PurposeTransient transfection into 293T cells is a convenient way to overexpress and obtain both cellular and extracellular (secreted or membrane) pro
Author: Nanci DonackiSource: Contributed by Nanci DonackiAbstract: Procedure for establishing hybridoma in one stepReagents(StemCell Te
ReagentsMedium A - Pre-fusion Medium and Hybridoma Expansion MediumMedium B - Fusion Medium Medium C - Hybridoma Recovery MediumMedium D - Hybridoma S
實驗概要將轉移基因整合到細胞染色體DNA上,形成穩定表達轉移基因的細胞系。 實驗原理 細胞轉染技術是目前廣泛應用于病毒基因結構與功能以及基因調控等的研究。細胞轉染可分為短暫轉染和穩定(或永久) 轉染兩種。在短暫轉染中,被轉染基因并不整合至細胞染色體中,因而不能隨細
常規操作(主要內容如下)· Aseptic Technique· Culture Ves
人胚腎 293 (HEK293) 細胞在重組蛋白表達中是最常見的宿主細胞。 這類細胞能夠表達大量的膜蛋白，如 G 蛋白偶聯受體 (GPCR) ，是無法在最常見的生物制藥生產宿主，如：中國倉鼠卵巢 (CHO) 細胞中作表達。 HEK293 雖然是蛋白表達的極好宿主，然而 H
Seeding After cells are thawed:NOTE: Do not dispense the entire contents of the cryovial into one T-25 flask!!Remove the cap, being car
實驗概要Adenoviral vectors widely used to transfer foreign genes into neuronal cells possess tropism for glial cells and are toxic to infected cells.
3. If your result falls into any quadrant other than the "High Yield-High Viability" quadrant, refer to Appendix D, Improving Cell Yield and
IV. MAINTENANCECultures should be examined daily, observing the morphology, the color of the medium and the density of the cells. A tissue culture log
Materials and MethodsCell culture conditions and surface transitionCryopreserved hiPSCs (SC102A-1, SBI?, USA) were initially thawed and pre-cultivat
REFERENCES:R. Ian Freshney, Culture of Animal cells: A manual of basic techniques, Wiley-Liss, 1987.VI. TISSUE CULTURE PROCEDURESEach s
The hepatocyte growth factor receptor, also called c-Met, is activated by HGF and stimulates proliferation of hepatocytes and other cell types. Mutate
Hematopoietic Stem Cell Targeting with Surface-Engineered Lentiviral VectorsEls Verhoeyen and Fran?ois-Lo?c CossetAdapted from Gene Tra
選擇何種稀釋液稀釋DNA及轉染試劑，對于制備有效的轉染復合物至關重要。除了溫度，孵育時間外，稀釋液的性質對于制備高效的轉染復合物亦非常重要，同樣影響DNA轉染效率。根據我們的實驗數據，使用合適的稀釋液得到的轉染效率是使用錯誤稀釋液轉染效率的至少50倍。更為重要的是，實驗者總是忽視稀釋液的重要性，甚至
Tissue collection1. Endometrial biopsies were collected from women undergoing gynaecological procedures for benign conditions. 2. All wome
Maintenance of Cell CultureAuthor: Nanci DonackiSource: Contributed by Nanci DonackiDate Added: Tue May 14 2002Date Modified: Tue
Growth and storage of Agrobacterium tumefaciensStrain GV3101: resistant to gentamycin and rifampicin so add 25-50 ug/ml Gentamycin, 10 ug/ ml rifampic
Figure 5: Regulation of CDH5 by TFZFs in several human cancer cell lines.Blue, cells infected with a pMX construct containing the DNA bindin
實驗概要Live cell studies of cellular DNA content and cell cycle distribution are useful to detect variations of growth patterns due to a vari
實驗概要Live cell studies of cellular DNA content and cell cycle distribution are useful to detect variations of growth patterns due to a vari
Electroporation ProtocolPreparation of Electro-competent Cells:1. Grow XL1-Blue cells on a tetracycline plate (20 ug tet/ml of LB agar)2. Inoculate 3
DNA轉染· Transfection of Mammalian Cells Using Lipofectamine (LTI)· &n
TROUBLESHOOTINGProblem: The OP9 cells are more than 80%-90% confluent.Solution: It is important when creating working stocks of OP9 cel
If culturing in the absence of a feeder cell layer is desired, human embryonic stem (hES) cells can be maintained using Mouse or Human-Conditioned Med