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REFERENCES:R. Ian Freshney, Culture of Animal cells: A manual of basic techniques, Wiley-Liss, 1987.VI. TISSUE CULTURE PROCEDURESEach student should maintain his own cells throughout the course of the experiment. These cells should be monitored daily for morphology and growth characteristics, fed every 2 to 3 days, and subcultured when necessary. A minimum of two 25 cm2 flasks should be carri......閱讀全文
AimDNA staining methods such as Hoechst staining techniques are quick with results available within 24 hours, which compares favorably with 4 weeks fo
· Cell Culture Media and Solutions (Donis-Keller lab)· &n
Working Cell Bank (Contributed by Nanci Donacki)Provides detailed protocol for establishing a working cell bank Maste
實驗概要The thymidine analog, 5-bromo-2-deoxyuridine (BrdU),is a common reagent used for cell proliferation assays and for the detection of apoptotic
常規操作(主要內容如下)· Aseptic Technique· Culture Ves
Handling Precautions Normal human cells are fragile, and require special handling:Upon receipt, immediately store cryopreserved cells i
PRIMARY MOUSE KERATINOCYTE CULTURESIsolation of epidermal keratinocytes from neonatal mice is based on the protocol of Dlugosz et al., Methods En
AbstractFour methods (an enzyme activity assay, western blotting, RT-PCR, and northern hybridization) to detect the enzyme D-amino-acid oxidase are de
1. Surface epithelial cells from normal ovaries from surgical residual specimen were isolated using standard and Institutional Review (Refere
3. If your result falls into any quadrant other than the "High Yield-High Viability" quadrant, refer to Appendix D, Improving Cell Yield and
實驗概要E.Z.N.A.? Total RNA Midiprep Kit provides a rapid and easy method for the isolation of up to 600 ug of total RNA from cultured eukaryo
Early in embryonic development, the region of the chick embryo which is determined to form a limb first differentiates from the rest of the embryo1. T
Lentivirus Transduction of Hematopoietic CellsMing-Jie Li and John J. RossiDivision of Molecular Biology, Beckman Research Institute of the City of Ho
Fine Mapping of Genomic Targets of Nuclear Proteins in Cultured CellsAchim Breiling and Valerio OrlandoDulbecco Telethon Institute, Institute of Genet
Mammalian RNA InterferenceThomas TuschlLaboratory for RNA Molecular BiologyThe Rockefeller University, New York Excerpted from RNAi: A Guide
Application: Quantitative RT-PCR is used to quantify mRNA in both relative and absolute terms. It can be applied for the quantification of m
Immunofluorescencc Microscopy of tissue culture cells (Microscopy and Electronic Imaging Lab)These methods are written for direct staining of fil
實驗概要Neural stem cells (NSC) are valuable resources because of their ability to differentiate into neurons and glial cells with application
實驗概要 A breakthrough in Arabidopsis research was the invention ofthe vacuum-infiltration procedure, a simple and reliabl
MaterialsMilipore filter type HA 0.45 micronCulture plates (Linbro model 76-033-05)Protein in DDW or HEPES buffer (10 mg/mL)Vacuum greaseSyringe with
Immunofluorescence Microscopy of tissue culture cellsThese methods are written for direct staining of filamentous actin with bodipy FL-phallicidin and
IV. MAINTENANCECultures should be examined daily, observing the morphology, the color of the medium and the density of the cells. A tissue culture log
NUCLEAR EXTRACTION OF TISSUE CELLSThis procedure was developed for HNEK cells grown in KGM, from Clonetics. All steps are performed on ice, with
Metabolic biotinylation of mammalian cell receptors for imagingBakhos A. Tannous , btannous@hms.harvard.edu, Massachusetts General Hospital and Harvar
9. Spontaneous germination. Some genotypes result in spontaneous ascospore germination (for example, the unpigmented ascospores of per-1 Type I). A si
Preparation of nucleic acid probesIn standard nucleic acid hybridization assays the probe is labeled in some way. Nucleic acid probes may be made as s
Bone marrow contains three types of stem cells:1. Hematopoietic stem cells give rise to the three classes of blood cells that are found in the
(Modification of methods of Dr. Seong-Seng Tan and Promega's "Protocols and Applications Guide")Cells previously transfected with a lac
由于細胞異質性的存在，單細胞層面的分析就變得十分重要。目前對于單細胞分析的方法主要還是通過化學、生物學的方法進行裂解后，提取內容物進行分析，然而這種方法往往會對樣本造成一些損傷。直接提取活細胞具有諸多優點，但是操作苦難。如今一種全新使用FluidFM科技的技術新報道有望提供一種活細胞提取新型的簡易方
DNA轉染· Transfection of Mammalian Cells Using Lipofectamine (LTI)· &n