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ConclusionIn summary, using comprehensive discovery proteomics analysis followed by target proteomics validation at a large scale sample size, we performed a system-based high throughput analysis to better understand the biological response induced by cryo-thermal therapy and reveal the stimulation of tumor-specific immunity at the molecular level. The cryo-thermal therapy induced strongest “acute” respon......閱讀全文
Interleukin-6 Induced Acute Phenotypic Microenvironment Promotes Th1 Anti-Tumor ImmunityTing Xue1, Ping Liu1, Yong Zhou2, Kun Liu1, Li Yang2, Robert L
In this study, we found that “acute” response was strongly induced by cryo-thermal therapy with “acute” and high expression of a series of acute p
The strongest and unique “acute” environment induced by cryo-thermal therapy stimulated the most effective anti-tumor immune response As desc
References1. Torre LA, Bray F, Siegel RL, Ferlay J, Lortet-Tieulent J, Jemal A. Global cancer statistics, 2012. CA: a cancer journal for clinician
IntroductionFetal Alcohol Syndrome (FAS) is caused by exposure of the developing embryo to alcohol, one of several teratogenic agents which adversely
As a primary component of circulatory system, serum is the major reservoir of thousands of proteins secreted or “leaked” from a broad spectrum of
Cryo-thermal therapy induced an “acute” phase response First, we performed PRM analysis upon significant proteins involved in acute phase respo
AbstractPrevious studies by Stachel and colleagues indicate that lithium chloride induction of post-midblastular Brachydanio rerio embryos r
In summary, we suggested that “acute” response could elicit innate and Th1 adaptive immunity, reversing the tumor-mediated immunosuppressive envir
Flow cytometrySpleens were extracted after therapy and mechanically homogenized with gentle MACS Dissociator to obtain a single-cell splenocyte su
Ambion and Applied Biosystems have joined forces to provide a complete convenient, solution for performing gene silencing experiments and validating t
在免疫學實驗中，比如WB，IHC，IF等，大家都知道選著好正確的一抗，往往實驗就成功了一大半，因此，往往很多研究者便會忽視對二抗，以及其它試劑盒品質的追求。二抗是一種相對而言比較穩定的產品，包裝大，因此很多實驗室都會公用一個二抗。但如果二抗沒有選擇好的話，影響的可能就是一整個實驗室或者課題組了。艾美
Figure 2. Silencer ? siRNA Validation Data Generated Using Applied Biosystems TaqMan? Gene Expression Assays. The indicated Silencer Validated siRNAs
血液腫瘤主要包括各類白血病、多發性骨髓瘤以及惡性淋巴瘤。急性白血病占常見惡性腫瘤的第八位，淋巴瘤也是在前十位，并且發病率逐年升高，多發性骨髓瘤的整個發病率在血液惡性腫瘤里面占10%。 血液腫瘤發病急，在傳統的放療、化療、骨髓移植等治療方式下，治療周期長、費用高昂、過程痛苦，但治愈率卻并不很
摘要：目的 研究中藥藥對虎杖-桂枝對急性痛風性關節炎的影響。方法 取雄性 SD 大鼠 48 只，隨機分為 6組（每組 8 只），即正常對照組，模型組，秋水仙堿組（0.1 g·kg-）1，虎杖-桂枝低、中、高劑量組（3.5，7，14 g·kg-）1。正常對照組和模型組給予等量生理鹽水，其他各組給予
二、細胞實驗效果不錯，那動物實驗怎么做？穩定性、重復性較好的動物模型可在腫瘤基礎性研究和抗腫瘤藥物篩選中發揮重要作用。血液腫瘤常用的動物模型主要有：I皮下移植瘤模型；II血液模型。皮下移植瘤模型較容易建立，易于動態觀察腫瘤的生長狀態。血液模型則一般以生存天數作為觀察終點，用活體成像、病理切片、流式細
摘要：目的 研究中藥藥對虎杖-桂枝對急性痛風性關節炎的影響。方法 取雄性 SD 大鼠 48 只，隨機分為 6組（每組 8 只），即正常對照組，模型組，秋水仙堿組（0.1 g·kg-）1，虎杖-桂枝低、中、高劑量組（3.5，7，14 g·kg-）1。正常對照組和模型組給予等量生理鹽水，其他各組給予相
摘要：目的 研究中藥藥對虎杖-桂枝對急性痛風性關節炎的影響。方法 取雄性 SD 大鼠 48 只，隨機分為 6組（每組 8 只），即正常對照組，模型組，秋水仙堿組（0.1 g·kg-）1，虎杖-桂枝低、中、高劑量組（3.5，7，14 g·kg-）1。正常對照組和模型組給予等量生理鹽水，其他各組給予
Circulating bone marrow (BM)–derived endothelial progenitor cells (EPCs) are recruited to the site of tissue regeneration and substantially contri
動物術中和術后保溫是動物實驗過程中的一個至關重要的環節，是否做好保溫措施直接關系到實驗的成敗，同時也是科研工作者在進行生命科學相關研究中將實驗動物倫理和福利付諸實踐的真正體現，是對動物愛心的社會責任感的體現。善待活著的動物，減少動物死亡的痛苦！動物手術保溫的科學價值Responsible scien
The PML nuclear bodies are ring-shaped nuclear substructures associated with the regulation of transcription, transformation, cell growth, and apoptos
The complement cascade of proteolytic factors involved in cellular lysis can be initiated by several different factors, including antibody-dependent a
Use of Expired MaterialsExpired medical materials such as drugs, fluids and sutures may not be used on any research animal who is unanesthetized
Mammalian cells can respond to a variety of stresses such as heat, cold, oxidative stress, metabolic disturbance, and environmental toxins through nec
Mario Roederer博士的團隊通過研究單細胞來更好地描述在HIV治療過程中免疫系統的特征。該團隊使用非人類靈長目模式動物，用單細胞基因表達分析提高T細胞的鑒定。他們使用了先進的技術，包括18色 FACS系統和Fluidigm BioMark? 系統進行單細胞基因表達分析。基本研究內容包括：
Finite element computational modeling of the resistive heating during the maximum exposure (150 V, 100 μs/phase, 10 Hz, 30 s) de
來自上海交通大學醫學院（SJTU-SM），中科院上海生命科學研究院健康科學研究院（Institute of Health Science），細胞分化與凋亡教育部重點實驗室（Key Laboratory of Cell Differentiation and Apoptosis of Chinese
Figure 2. Proteomics analysis of serum glycoproteins from treated and untreated mice. 21 days after cell injection, mice underwent cryo-
血糖監測是糖尿病管理的重要內容。已有多項大型臨床研究證實，糖化血紅蛋白（HbA1c）與糖尿病慢性并發癥的發生存在關聯，將HbA1c水平控制在合適的范圍內可以有效降低慢性并發癥的風險，因此，長久以來HbA1c一直被視作評價糖尿病患者長期血糖控制狀況的金標準[1]。然而，血紅蛋白的更新速度異常，某些情況
化合物對心肌細胞跳動的峰值參數的影響用FLIPR Tetra系統在化合物添加24小時后進行鈣振蕩信號的檢測和記錄。iPSC2s與之前版本的iPSCs以相同的方式進行實驗，通過形成縫隙連接并同步振蕩，檢測到鈣敏感染料指示的峰值頻率。新型細胞比之前版本的細胞可提早4-5天用于檢測實驗，同時因其更低的