ExperimentalSurgery2
Use of Expired MaterialsExpired medical materials such as drugs, fluids and sutures may not be used on any research animal who is unanesthetized or who is to recover from an anesthestic procedure. The use of such materials under these conditions constitutes inadequate veterinary care under the Animal Welfare Act. The IACUC has established the following guidelines for the use of expired medical materials:&nb......閱讀全文
Experimental－Surgery
General Issues and RequirementsSurgery is defined as any procedure that exposes tissues normally covered by skin or mucosa.? Experimental surgery has
Experimental－Surgery3
Antibiotic Guidelines General Recommendations Selection If a culture and sensitivity is not available, select antibiotics based on probable orga
Experimental－Surgery2
Use of?Expired MaterialsExpired medical materials such as drugs, fluids and sutures may not be used on any research animal who is unanesthetized or wh
Guidelines－for－Aseptic－Rodent－Survival－Surgery
Introduction:Aseptic surgery is surgery performed without contamination or exposure to pathogens. These policies and guidelines are provided to help e
Experimental－Protocol－for－cDNA－Library－Construction
Experimental Protocol for cDNA Library ConstructionIdentify appropriate celltype over-expressing corresponding gene.Find out if transcription can be s
Yale－University－Policy－for－Aseptic－Amphibian－Survival－Surgery
Background:?Aseptic surgery by definition is surgery performed without contamination or exposure to pathogens. According to the AWR's and the?Guid
Apoptosis:－A－Laboratory－Manual－of－Experimental－Methods－Andrea－Cossarizza
THE CELL?1.?Morphological aspects of apoptosis?Walter Malorni, Stefano Fais & Carla Fiorentini?2.?Cell cycle?Miriam Capri & Daniela BarbieriTHE NUCLEU
Experimental－Brain－Research：雙腿平衡研究新發現
2015年12月30日訊/生物谷BIOON/眾所周知，用雙腿走路本來就不穩定，這意味著人的中樞神經系統必須努力保持平衡。步行運動是由兩種前饋控制法來控制的，在步行期間出現可能干擾行走的穩定性時身體已經做出反應，當身體對一種未知的干擾產生反應而保持直立時就會得到反饋控制。這些控制方法的精確機制目前
JAMA－Surgery：減肥術治療2型糖尿病
近日，發表在《JAMA Surgery》的一項研究表明， Annemarie G. Hirsch博士和他的同事們研究了DiaRem得分（經過驗證的分數是來自比較易得的數據），DiaRem得分可以用于預測經過減肥手術的病人是否會促使2型糖尿病治愈。這個分數可以用來預測減肥手術是否會促使短期緩解糖尿
Experimental－Neurology：如何緩解中風帶來的大腦損傷？
中風患者如果其免疫細胞進入大腦，則會導致疾病的惡化。來自羅徹斯特大學醫學院的研究者們目前正視圖利用肺臟調節機體的免疫反應，進而緩解中風后的二次腦損傷的嚴重程度。 "我們越來越清楚肺臟是調節機體免疫系統的重要部位，因此能夠作為治療中風二次損傷的潛在靶點"，來自URMC的神經學家Marc Halt
基本無菌化技術
INTRODUCTION The regulations promulgated to implement the amended Animal Welfare Act require that all survival surgery be performed using aseptic pr
Principles－of－Aseptic－Technique
INTRODUCTIONThe regulations promulgated to implement the amended Animal Welfare Act require that all survival surgery be performed using aseptic proce
基本無菌化技術4
P REPARATION OF THE SURGEONIn a laboratory setting, the extent of surgeon preparation will depend on the facilities and the need for strict attention
Developmental－and－phenotypic－implications－of－transplantation
ObjectiveIn this experiment, a region of the neural crest will be transplanted from one stage-fourteen embryo to another (Figure 1). To facilitate the
Isolation－of－human－prostatic－smooth－muscle－cells
Human prostate tissues1.Human hyperplastic prostates were obtained during surgery from four men through transurethral resection of the prostate. 2.All
12篇Lancet/Nature文章被撤回，這項“顛覆性”的技術，為何成為殺人工具？
2011年，使用人工合成POSS-PCU納米復合結構體植入自體干細胞，進行了首例組織工程氣管-支氣管移植物的人體成功移植。 2012年8月16日，Paolo Macchiarini團隊在Biomaterials（IF=14）在線發表題為“Viability and proliferation
破冰行動：提速DNA電泳速度
? 4、 電源電壓 在低電壓時，線狀DNA片段的遷移速率與所加電壓成正比。但是隨著電場強度的增加，不同分子量的DNA片段的遷移率將以不同的幅度增長，片段越大，因場強升高引起的遷移率升高幅度也越大，因此電壓增加，瓊脂糖凝膠的有效分離范圍將縮小。要使大于2kb的DNA片段的分辨率達到最大，所加電壓
miRNA的表達與肺鱗癌的診斷與預后
世界上每年有超過160萬例肺癌新發病例，超過137萬人死于肺癌。中國每年有52萬例肺癌新發病例，超過45萬人死于肺癌。80%肺癌是非小細胞肺癌，非小細胞肺癌中40%是鱗癌。ⅠA期肺鱗癌患者的5年存活率約為60%，而Ⅱ-Ⅳ期肺鱗癌患者的5年存活率為5% - 40%。因此，發現新的標志物并應用于早期
自由基快速檢測分析儀應用：氧化應激與主動脈手術后...
自由基快速檢測分析儀應用：氧化應激與主動脈手術后肌肉萎縮具有相關性Redoxsys自由基快速檢測分析儀應用性論文Oxidative stress is associated with muscle wasting after aortic surgery（氧化應激與主動脈手術后肌肉萎縮具有相關性）R
中科院發布期刊分區表！附一區二區期刊清單
醫學CA-A CANCER JOURNAL FOR CLINICIANS1189.734NEW ENGLAND JOURNAL OF MEDICINE170.408NATURE REVIEWS DRUG DISCOVERY151.429LANCET148.362JAMA-JOURNAL OF THE
Principles－of－Aseptic－Technique——4
P REPARATION OF THE SURGEONIn a laboratory setting, the extent of surgeon preparation will depend on the facilities and the need for strict attention
Lasers－Surg－Med：低劑量光動力學療法可以調控腫瘤微環境
一項新的研究揭示了低劑量光動力療法影響血管微結構的機制。通過體外共培養周細胞和內皮細胞，研究人員發現低劑量光動力療法通過Rho、肌球蛋白輕鏈和局灶性粘附激酶磷酸化(MLC‐P, FAK‐P)來激活周細胞。這導致細胞骨架重構，導致周細胞更有效地收縮三維膠原凝膠。圖片來源：Laser in Surg
Development－of－the－Heart－Morphogenetic－Field－in－the－Axolotl－Embryo
IntroductionThere are two modes of development common to most species in the animal kingdom.Virtually all embryos undergo both mosaic and regulative d
FGFR－INHIBITOR,－SU5402,－ON－CRANIOFACIAL－DEVELOPMENT－IN－CHICKEN－EMBRYOS
ABSTRACTFibroblast growth factor (FGF) signaling plays a signifigant role in embryonic development. Particularyl, FGFs are implicated in mediating epi
Investigating－the－effect－ofthe－teratogen－cyclopamine－on－Ambystoma－mexicanum
ObjectiveThis experiment attempted to determine the effects of the teratogen cyclopamine on the Shh signaling pathway in axolotl embryos. Groups of ax
LaVision雙光子顯微鏡亞細胞水平的深層組織成像（五）
結論??? 這些結果表明紅外雙光子和二次諧波產生顯微成像對于無毒害的時間分辨的細胞行為調查的深層組織成像尤其有利。作為與其它脈沖飛秒激光系統相比的優勢，如Ch:forsterite (1230 nm) 和 Fianium fiber (1064 nm) 激光器，OPO產生的波長是可調諧的
CRISPR基因組編輯有望用于整形外科中
CRISPR基因組編輯技術有望成為基因工程和治療的“變革性飛躍”，它幾乎影響到醫學的每個領域。根據2018年11月發表在美國整形外科學會（American Society of Plastic Surgeon）官方期刊Plastic and Reconstructive Surgery上的一篇標
Separation－of－sea－urchin－blastomeres
Objective: to separate the Blastomere at different points in development and observe the growth of the resulting embryos. Procedure:?We extractrf th
PrestoBlue?－Cell－Viability－Reagent－Protocol
實驗概要PrestoBlue? Cell ?Viability Reagent is a ready‐to‐use reagent for rapidly evaluating the ?viability and proliferation of a wide range of cell type
辛迪思有限公司－Synthes－GmbH－對創傷外科手術器械包主動召回
強生（上海）醫療器材有限公司報告，由于螺絲刀軸桿頂端凹槽未進行加工，生產商辛迪思有限公司 Synthes GmbH 對其生產的創傷外科手術器械包Instruments for Trauma Surgery （國械備20180944號）主動召回。召回級別為三級召回。涉及產品的型號、規格及批次等詳細