Method:PreparationofLymphoblastoidCellLinesforLongTermStorage
Method: Preparation of Lymphoblastoid Cell Lines for Long Term StorageMay 30, 1990Rosalie VeilePurpose:To store cell lines in a form that will insure recovery with high viability.A culture in logarithmic phase of growth with a total volume of 80-100 ml/T-75 flask should yield enough cells to freeze 10 ampules (1.0 ml/ampule). Cells should have a count of 4 X 106 cells/ampule to 9 X 106 cells/ampule. Too high or too l......閱讀全文
Method: Preparation of Lymphoblastoid Cell Lines for Long Term Storage
Method: Preparation of Lymphoblastoid Cell Lines for Long Term Storage May 30, 1990 Rosalie Veile Purpose: To store cell lines in a form
Method: Maintaining Lymphoblastoid Cell Lines
Method: Maintaining Lymphoblastoid Cell Lines June 10, 1990 Rosalie Veile Purpose: To grow lymphoblastoid cells for permanent stora
巨噬細胞和單核白細胞
·?????????Lymphocyte Transformation?(Donis-Keller lab)Lymphocytes are transformed to establish cell lines. Mononuclear cells (lymphocytes) from antico
Long Term Storage of Bacterial Strains
Purpose:Bacterial strains may be stored indefinitely at low temperatures (- 20 degrees C and -80 degrees C) in 15 to 40 glycerol. It is lab policy to
Method: Preparation of Lymphocyte Cell Pellet for Storage
Method: Preparation of Lymphocyte Cell Pellet for Storage June 10, 1990 Rosalie Veile Purpose: Following propagation to 1 X 108 cells, l
Method: Lymphoblastoid Cell Lines from Frozen Whole Blood
Method: Lymphoblastoid Cell Lines from Frozen Whole Blood May 31, 1990 Rosalie Veile Purpose: Blood Samples can be stored frozen as a
細胞培養常規操作
常規操作(主要內容如下)·?????????Aseptic Technique·?????????Culture Vessels·?????????Cell Counting·?????????Primary Culture·?????????Maintenance of Cell Line?·??
Method: Reactivating Cell Lines and Cell Growth for DNA Preparation
Purpose:Cell lines are reactivated and grown to a count of 1 x 108 cells. The cells are pelleted and stored frozen at -80 degrees C prior to DNA extra
Long Term Storage of Transformed E.coli
Long Term Storage of Transformed E.coliTransfer 10 ml of 250 ml overnight culture in sterile flip-cap 15 ml Tube.Add sterile Glycerol to 15 % final co
細胞培養——細胞保藏
Working Cell Bank?(Contributed by?Nanci Donacki)Provides detailed protocol for establishing a working cell bank????Master Cell Bank?(Contributed by?Na