Matrigelinvasionassays
OverviewMatirgel is considered as basement membrane and generated from EHS sarcoma. Matrigel contains not only basement membrane components (collagens, laminin, and proteoglycans)but also matrix degrading enzymes/their inhibitors and growth factors. Invasion of tumor cells into Matrigel has been used to characterize involvement of ECM receptors and matrix degrading enzymes which play roles in tumor progression. ......閱讀全文
Matrigel invasion assays
OverviewMatirgel is considered as basement membrane and generated from EHS sarcoma. Matrigel contains not only basement membrane components (collagens
Agglutination Assays
Agglutination Assays REFERENCE:?Lanyi, B., and T. Bergan. Methods in? Microbiology, Vol 10: 93-168.? BACTERIAL AGGLUTINATION:? Bacterial agglutinat
Carbohydrate Assays
Carbohydrate Assays REFERENCE:?Wright and Rebers, Anal. Biochem. 49: 307-319, 1972. OBJECTIVE:?To determine the relative amounts of LPS carbohydrat
Cellulase assays
實驗概要 ? ? ? ? Cellulase enzymes show activity during the ripening of some fruits, where their effects on cell walls results in softening of the f
Cellulase assays
Cellulase enzymes show activity during the ripening of some fruits, where their effects on cell walls results in softening of the fruit. In cases of p
Microtubule Binding Assays
MaterialsSiliconized ultracentrifuge microfuge tubesGTP-depleted microtubules6X SDS loading dye1X SDS loading dyeCoomassie Brilliant Blue R 250 (0.8%
Cytotoxicity Assays Protocol
Cytotoxicity Assays ProtocolCell-mediated cytotoxicity was determined by using a standard microcytotoxicity assay. Briefly, target cells were pelleted
SAPK/Jun kinase assays
Preparation of cell lysate:1. For cells in suspension, grow in DMEM with 10% FCS and then the day before you do the experiment spin the cells down (1
DNA Fragmentation Assays for Apoptosis
Protocol I:?Triton X-100 Lysis BufferIn 96 flat-wells plate, incubate 4x10 6 target cells (40 wells of 105 per well) with desired concentration of eff
PLAQUE ASSAYS FOR ADENOVIRUS TITRATION
-Set up 60 mm dishes of P11 cells to be 100 confluent at time of infection.?-Remove medium from dishes, add 0.2 to 0.5 ml virus and adsorb for 30 – 60