ExaminationofaMammalianBloodSmear
Examination of a Mammalian Blood SmearThe distribution and appearance of the formed elements of blood can tell a great deal about the condition of the donor. Disproportionate numbers of white cells (leukocytes) and/or the presence of immature leukocytes are indications of a serious disease, as are high or low platelet counts or misshapen red blood cells. The presence of a high proportion of eosinophils (see figure be......閱讀全文
Examination－of－a－Mammalian－Blood－Smear
Examination of a Mammalian Blood SmearThe distribution and appearance of the formed elements of blood can tell a great deal about the condition of the
Blood－Smear:－Preparation－and－Staining
Blood Smear: Preparation and StainingReference:Davidson, I. and Henry J., Clinical Diagnosis by Laboratory Methods, I. Davidsohn and J. Henry, eds., W
SMEAR－PREPARATION
The preparation of a smear is required for many laboratory procedures, including the Gram-stain. The purpose of making a smear is to fix the bacteria
Freezing－and－Thawing－of－Mammalian－Cell－Lines
For long term storage of myeloma cells, hybridoma cells, T cells, and other mammalian cell lines in liquid nitrogen, and restoring them in culture.
Transfection－of－Mammalian－Cells－Using－Lipofectamine
Materials:??????? Lipofectamine Basal Medium containing 10% fetal bovine serum, 1% glutamine, 1% aa Basal Medium containing 1% glutamine
Cellfree－System－for－the－examination－of－apoptotic－activity
?IntroductionIn our lab we use the term 'cell-free system' when we talk about the examination of apoptotic activity in cytoplasmic extracts. T
Steps－in－the－Glycosylation－of－Mammalian－Nlinked－Oligosaccarides
The biosynthesis pathway of N-glycans is a costly system with respect to the number of enzymes that are involved in the synthesis and trimming of N-gl
RNA－analysis－on－nondenaturing－agarose－gel－electrophoresis
1. The following gel electrophoresis conditions are recommended: - use 1X TAE buffer instead of 1X TBE - use agarose gel in the concentration o
RNA－analysis－on－nondenaturing－agarose－gel－electrophoresis
實驗概要RNA analysis on non-denaturing agarose gel electrophoresis實驗步驟1. The following gel electrophoresis conditions are recommended:- use 1X TAE buffer
Protocol－for－Isolating－DNA－from－Blood－with－Nucleated－Red－blood－Cells
實驗概要 DNA ?isolation from fish or avian blood sample can be difficult because it ?contains nucleated red blood cells. E.Z.N.A. NRBC Blood DNA Kit is
SQ－Blood－Mini－Protocol－for－1ml－Clotted－Blood
實驗概要 This protocol is designed for isolating genomic DNA from 1ml Clotted Blood. 主要試劑 Regents to be supplied by user 1. Proteinase K (20mg/ml) 2
SQ－Blood－Mini－Protocol－for－50ul－Clotted－Blood
實驗概要 This protocol is designed for isolating genomic DNA from 0.5-1 million cultured cells. For larger or smaller amounts of starting cell numbe
Lineage－Analysis－of－Blood
Materials:Capillary tubes1.5 mL Eppendorf microfuge tubes15 mL conical centrifuge tubes96-well V-bottom plates (Corning Costar 3894, from Fisher)Flow
哺乳動物RNAi技術－Mammalian－RNA－Interference
Mammalian RNA InterferenceThomas TuschlLaboratory for RNA Molecular BiologyThe Rockefeller University, New York?Excerpted from?RNAi: A Guide To Gene S
PCR儀產生彌散（smear）條帶原因分析
*在PCR反應體系中第一鏈產物的含量過高 *減少引物的用量 *優化PCR反應條件/減少PCR的循環次數 *在用DNase處理被DNA污染的RNA樣品時，其產生的寡核苷酸片段會產生非特異性擴增，一般會顯示為彌散背景。
SQ－Blood－DNA－Maxi－Protocol－for－410－ml－whole－blood
實驗概要 The E.Z.N.A.? ?SQ Blood DNA Kit is designed for isolating high molecular weight ?genomic DNA from fresh, frozen or anticoagulated whole blood.
陰道分泌物涂片檢查正常參考值及臨床意義
中文名稱：陰道分泌物涂片檢查 英文名稱：Microscopic Examination of Endocrine Function in Vaginal Smear 正常參考值：清潔度Ⅰ—Ⅱ度，不見滴蟲、霉菌。 臨床意義： 膿性白帶：見于滴蟲性陰道炎、慢性宮頸炎、老年性陰道炎、子宮內
Mouse－Compete－Blood－Counts
Materials:? 250 μL of fresh mouse blood in plastic tubes containing EDTA.? RBC lysis buffer?(388 mM NH4Cl, 29.7 mM NaHCO3, 25 μM Na2EDTA) 20.
DNA－Extraction－from－Blood
實驗概要The ChargeSwitch? ?gDNA Purification Kits allow rapid and efficient purification of ?genomic DNA from small volumes of human blood. After preparin
Rat－Blood－Collection－Protocols
實驗概要The procedure presented below describes a method for collecting rat blood.實驗步驟Rat should be fully anesthetized (e.g., unresponsive to toe pinch).1
SQ－Blood－DNA－Midi－Protocol－for－500l3ml－whole－blood
實驗概要 The E.Z.N.A.? ?SQ Blood DNA Kit is designed for isolating high molecular weight ?genomic DNA from fresh, frozen or anticoagulated whole blood.
醫學“偵查兵”的秘密（20）
近年來，檢驗醫學發展迅速。為了方便體外診斷產業界的同仁們了解和掌握日常檢驗的中英文名稱、正常值參考范圍和簡要臨床意義，我會將每天編輯推送3條相關訊息，以饗讀者。 1、前列腺液涂片檢查 中文名稱：前列腺液涂片檢查 英文名稱：Microscopic Examination’
Serum－Separation－from－Whole－Blood
Serum Separation from Whole Blood 1) Collect sample (preferably in glass tubes) and leave for 1 hour at 37°C to allow it to clot. 2) Leave sample a
Separation－of－Platelets－from－Whole－Blood
Purpose This protocol describes how to isolate human platelets from whole blood. Isolated platelets are used for static adhesion assays, for flow ch
Harvesting－lymphocytes－from－Peripheral－Blood
Blood Harvest·??????? Anesthetize mice with 50ml of Xylene-Ketamine(2:1) coctail·??????? Pin animal to a board (use distal extremities) supine·???????
iPrep?－GeneCatcher?－gDNA－Blood－Kit
實驗概要The iPrep? ?GeneCatcher? gDNA Blood Kit allows rapid and automated extraction of ?genomic DNA (gDNA) from human blood including archived or poorly
Peripheral－blood－“endothelial－progenitor－cells”
EPC Isolation and Characterization1.?EPCs were obtained by isolating mononuclear cells using Ficoll density-gradient centrifugation of human blood buf
Red－Blood－Cell－Lysis－Protocols
實驗概要BioLegend’s ?Red Blood Cell (RBC) Lysis Buffer (Cat. No. 420301) has been designed, ?formulated, and tested to ensure optimal lysis of RBCs in sin
Microscopy－with－Oil－Immersion
Microscopy with Oil ImmersionPrincipleWhen light passes from a material of one refractive index to material of another, as from glass to air or from a
Human－Peripheral－Blood－Mononuclear－Cell－Preparation
This protocol describes a procedure for isolating human peripheral blood mononuclear cells (lymphocytes and monocytes) from a Buffy Coat (obtained fr