WIKI資訊
PurposeThis protocol describes how to isolate human platelets from whole blood. Isolated platelets are used for static adhesion assays, for flow chamber assays, flow cytometry measurements, etc.MaterialsCitrate buffer (25 g sodim citrate, 8 g citric acid, 500 ml H2O2).Prostaglandin E1 (PGE1).ACD buffer (6.25 g sodium citrate.2 H2O , 3.1 g citric acid anhidrous, 3.4 g D-glucose in 250 ml H2O).Hepes-Tyrode buffer ......閱讀全文
Examination of a Mammalian Blood SmearThe distribution and appearance of the formed elements of blood can tell a great deal about the condition of the
Sample preparation and solubilization are crucial factors for the overall performance of the 2-D PAGE technique. Protein complexes and aggregates shou
常規操作(主要內容如下)· Aseptic Technique· Culture Ves