WIKI資訊
BackgroundZebrafish, Brachydanio rerio, have a distinct embryonic development. The embryo goes through a process called epiboly. Epiboly, as defined in the sixth edition of Developmental Biology(Gilbert 2000), is the movement of epithelial sheets (usually of the ectoderm cells) that spread as a unit, rather than individually, to enclose the deeper layers of the embryo. Epiboly enables an embr......閱讀全文
Introduction: This method for the detection of cellular proliferation includes several modifications of a previously published protocol (Hayashi, et a
The siRNA user guide (revised May 6, 2004) Selection of siRNA duplexes from the target mRNA sequenceUsing Drosophila melanogaster lysa
Selection of siRNA duplexes from the target mRNA sequenceUsing Drosophila melanogaster lysates (Tuschl et al. 1999), we have systematically analyzed t
Introduction The most important aspect of our cloning vectors is that t
Metabolic biotinylation of mammalian cell receptors for imagingBakhos A. Tannous , btannous@hms.harvard.edu, Massachusetts General Hospital and Harvar
2.2 Interaction mating - large scaleWith a few modifications, the procedure described above can be used to test for interactions between a single prey
Researchers are usually trained in RNA isolation and analysis methods by one another or by technical manuals. Experimental procedures are often not qu
核心提示：關于牛奶中IGF-1對人體的影響，美國食品藥品局（FDA）早已經做出了結論。2007年5月，在FDA最新公布、經美國國家審計署審計的報告中寫道：“IGF-1與癌癥無關，牛奶中IGF-1濃度的變化不會增加人體罹患癌癥的幾率”。
圖3 H+和O2 流動速率的同時測量.(a)顯微照片顯示金屬氧電極與玻璃H+電極同時測量百合花粉管生長過程中H+離子和O2分子進出的變化；(b)在花粉管線粒體密集區域, 或稱固有堿化帶區域，同時存在的H+外流和O2內流現象. 2.2 SIET與熒光顯微技術結合證明
Application: Quantitative RT-PCR is used to quantify mRNA in both relative and absolute terms. It can be applied for the quantification of m
Lipoprotein Analysis Week 2: Electrophoresis IntroductionSDS polyacrylamide gel electrophoresis (SDS PAGE) will be used to assess the
Non-denaturing PAA gelsTo separate PCR products differing in only a few bp in length (for example, microsatellite markers), 6-10% PAA gels need to be
ABSTRACTFibroblast growth factor (FGF) signaling plays a signifigant role in embryonic development. Particularyl, FGFs are implicated in mediating epi
Sapphire Window Development and Testing.The effort of this work was devoted to fabricating ultrathin strengthened sapphire disks and to accurately
IntroductionIn our lab we use the term 'cell-free system' when we talk about the examination of apoptotic activity in cytoplasmic extrac
Stem cell di?erentiation assaysAdMSCs were harvested from both shake and spinner ?asks into 50 ml tubes. Once the microcarrier beads settled to the
3.2.1 LabelingThis method requires the starting material 3′-amino-3′-deoxy--ATP (15).1. Activate 7-diethylaminocoumarin-3-carboxylic
實驗概要 This protocol utilizes the powerful guanidine isothiocyanate–phenol:chloroform extraction method which allows the
I. Safety ProceduresA. ChemicalsA number of chemicals used in this laboratory are hazardous. All manufacturers of hazardous materials are require
實驗概要 The immunoprecipitation (IP) of cross-linked chromatin with antibodies specific for certain histone modifications (chromatin &nb
Once high molecular weight (HMW) DNA has been prepared it must somehow be fragmented and DNA in the desired size range isolated. In general, as the de
Immobilization of ligands on cuvette surfaces and measure the interactions of ligand which is immobilized on the cuvette and the ligate which is a
ObjectiveThe objective of the experiment is to determine the effects of ethanol exposure on the embryonic development of zebrafish through observation
Acknowledgements The organizer of the workshop acknowledges Dr. Murray Milford, Professor and Interim Head, and Dr. Mark Hussey, Professor and In
Joseph SambrookPeter Maccallum Cancer Institute and The University of Melbourne, AustraliaDavid W. RussellUniversity of Texas Southwestern Medical Cen
F. Video Microscopy 1. The behavior of migrating cells may be filmed with an inverted microscope fitted with a CCD camera. 2. Determine the
Objective The purpose of this experiment is to observe the effect of caffeine on the heart rate of three day old ch
Preparing Lambda DNA1- Coliphage lambda DNA is a widely used vector for recombinant DNA. The middle third of its 48,000 bp contains no genes required
Direct labeling of total RNA with Cy3 and Cy5:A. MATERIALSRNeasy? Mini Kit (Qiagen; Cat # 74106) SuperScript II RT (200U/μL) (Life Technologies;
DNA and RNA Staining6. Stain cells with 7-AAD: i. Resuspend the cells from Step 5 in 0.5 mL of NASS containing 10 μg/mL of 7-AAD. Incub