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3.2.1 LabelingThis method requires the starting material 3′-amino-3′-deoxy--ATP (15).1. Activate 7-diethylaminocoumarin-3-carboxylic acid (16.4 mg, 62.8 μmol) by dissolving in dry DMF (1 ml), cooling it on ice, and adding tributylamine (25 μl, 103 μmol) and isobutyl chloroformate (10 μl, 77 μmol).2. Leave the reaction mixture on ice for 50 min.3. Add 3′-amino-3′-deoxyATP (40 μmol, triethyla......閱讀全文
By: Christopher P. Toseland1 2 , Martin R. Webb1Affiliation(s): (1) MRC National Institute for Medical Research, London, UK(2
References1.Funatsu, T., Harada, Y., Tokunaga, M., Saito, K., and Yanagida, T. (1995) Imaging of single fluorescent molecules and individual ATP turno
1.1 Selection of the Labeling PositionFluorescent adenine and guanine nucleotides have been widely used to report upon binding, protein re
隨著人類基因組計劃（human genome project ）在2003年順利完成，基因組測序技術取得了長足的進步，這直接導致了每兆基因組成本的大幅下降以及檢測的基因組數量越來越多。人們對基因組的復雜性深感震驚，這也引導著測序技術的進一步發展。最近的一些突破性技術使得測序技術在更短的時間內可以
應用WGS正在成為NGS中最廣泛的應用。通過該技術并且結合生物學應用，研究人員可以獲得基因組信息中最值得注意的信息73。舉例來說，2012年，Ellis等報道了基因與乳腺癌患者芳香酶抑制劑（aromatase inhibitor）治療法之間的關聯。他們指出突變，后果與診斷之間的關聯，同樣還有
借助Chromotek公司GFP-Trap如何設計成功的IP實驗？How to plan an immunoprecipitation of your GFP-fusion protein when using the ChromoTek GFP-Trap?PreambleThis document
NGS 是一種識別和確認未知致病菌的前景廣闊的技術，然而其在生物防御和公共健康應用等方面的時效性，卻往往因為缺乏快速、有效、可靠的自動DNA樣品制備方法而受到限制。為了突破這種限制，Kim 等設計了一種基于流體分布元件的數字微流體(DMF) 平臺，使得多子系統模塊能夠進入自動NGS庫樣品
Fig. 6HS-AFM observations of the non-specific transient binding of Cas9–RNA. a Sequential HS-AFM images of Cas9–RNA molecules transiently bound to
Section 2.1Nondenaturing Polyacrylamide Gel Electrophoresis of ProteinsJohn M. Walker1. IntroductionSDS-PAGE (Section 2.2) is probably the most
BCEIA2019 International Summit on Analytical Instrumentation and In Vitro Diagnosis--Registration in ProgressIn recent years，In Vitro Diagnosis has be
多功能單細胞顯微操作系統FluidFM BOT在活細胞提取中的應用由于細胞異質性的存在，單細胞層面的分析就變得十分重要。目前對于單細胞分析的方法主要還是通過化學、生物學的方法進行裂解后，提取內容物進行分析，然而這種方法往往會對樣本造成一些損傷。直接提取活細胞具有諸多優點，但是操作苦難。如今一種全新使
由于細胞異質性的存在，單細胞層面的分析就變得十分重要。目前對于單細胞分析的方法主要還是通過化學、生物學的方法進行裂解后，提取內容物進行分析，然而這種方法往往會對樣本造成一些損傷。直接提取活細胞具有諸多優點，但是操作苦難。如今一種全新使用FluidFM科技的技術新報道有望提供一種活細胞提取新型的簡易方
還記得CRISPR-Cas9基因組編輯技術，cryo-EM，甚至高通量測序技術未出現之前，我們是怎樣進行研究的嗎？其實大家不用回憶太久，因為這不是很久以前的事。在過去幾年間，生物學研究技術進步步伐快的讓人難以置信。 Cell出版社旗下Molecular Cell雜志推出了技術特刊，介紹了新技術
實驗概要The LIVE/DEAD? Fixable Dead Cell Stain Kits use a novel method to evaluate the viability of mammalian cells by flow cytometry. These a
實驗概要The LIVE/DEAD? Fixable Dead Cell Stain Kits use a novel method to evaluate the viability of mammalian cells by flow cytometry. These a
In Vivo Imaging of Far-red Fluorescent Proteins after DNA Electrotransfer to Muscle TissueDNA electrotransfer to muscle tissue yields long-term, high
To determine the minimum dose of Katushka plasmid needed to give detectable fluorescent intensity, we decreased the amount of pTurboFP635 to 0.5 and 1
實驗概要Invitrogen offers several fluorescent and biotinylated phalloidin and phallacidin derivatives for labeling F-actin. These phallotoxins
實驗概要Invitrogen offers several fluorescent and biotinylated phalloidin and phallacidin derivatives for labeling F-actin. These phallotoxins
實驗概要Platinum? qPCR SuperMix for SNP Genotyping is a ready-to-use reaction mix for the amplification and identification of single-nucl
back to topProtocolStandard vs. Rapid Immunodetection ProceduresThere are two types of protocols for immunodetection: Standard and rapid.Standard vs.
IntroductionLive imaging provides an important complementation to the "snapshot" view obtained in fixed tissue by immunofluorescence. It all
Conjugation of monoclonal antibodiesPrelude: You are free to copy and distribute these documents at will--but please do so in their entirety, complete
A fluorescence-based in vitro assay for investigating early endosome dynamicsSina V Barysch1,2, Reinhard Jahn1 & Silvio O Rizzoli2A
Flow Cytometry Analysis (Springer Lab, Harvard University) Flow cytometry employs instrumentation that scans single cells flowing past excit
最方便的實驗干貨查詢工具微信掃碼進入「丁香實驗」小程序編輯： 嗚咽分享到： Flow Cytometry Analysis (Springer Lab, Harvard University)Flo
實驗概要We provide a guideline procedure and tips for staining of paraffin embedded sections, including antigen retrieval, chromogenic detecti
實驗概要The LIVE/DEAD? Violet Viability/Vitality Kit provides a two-color fluorescence cell viability and vitality assay that is based on the
實驗概要The LIVE/DEAD? Violet Viability/Vitality Kit provides a two-color fluorescence cell viability and vitality assay that is based on the
Background:Zebrafish,or the teleost fish Danio rerio,is a rapidly developing organism that is apopular species for studying vertebrate development. Cl