Objective: to test the effects of inhibiting the fast and slow blocks to polyspermy on sea urchin development.BackgroundSea urchin eggs release components of the egg jelly that attract sperm and induce the acrosome reaction (Gilbert, 2000). In the laboratory, a single egg many be covered with dozens or even hundreds of sperm burrowing through the egg jelly.Once a sperm contacts the viltelline membrane, it binds to a ......閱讀全文
FL3500雙調制葉綠素熒光儀 (新升級型號為FL6000) FL3500雙調制葉綠素熒光儀是專門用于對藍綠藻或綠藻等微藻,葉綠體或類囊體懸浮物,乃至葉片進行光合作用研究的強大科研工具。儀器具備雙通道測量控制,可控制測量樣品的溫度,并配備單翻轉光(STF),內置多種可用戶自行修改的測量程序
This method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor
Degenerate PCR is in most respects identical to ordinary PCR, but with one major difference. Instead of using specific PCR primers with a given sequen
What is degenerate PCR? Degenerate PCR is in most respect identical to ordinary PCR, but with one major difference. Instead of using
What is Anesthesia? Anesthesia is a state of unconsciousness induced in an animal. The three components of anesthesia are analgesia (pa
A few cell types are thin enough to be viewed directly in a microscope (algae, protozoa, blood, tissue cultures), but most tissues (kidney, liver, bra
BASIC THEORY AND USE OF GC-MSbyDr. Eugenia SobolevaContent1. Introduction.2. GC-MS syste
Differential Interference Contrast (Nomarski, DIC, Hoffman Modulation Contrast)PrincipleDifferential interference microscopy requires several optical
AbstractPrevious studies by Stachel and colleagues indicate that lithium chloride induction of post-midblastular Brachydanio rerio embryos r
A target-specific whole cell assay for antibacterial drug discoveryLorraine HernandezSrinivas KodaliDoris Cull
I. Safety ProceduresA. ChemicalsA number of chemicals used in this laboratory are hazardous. All manufacturers of hazardous materials are require
Four different members of the Sprouty protein family block the cellular proliferation and differentiation induced by several different growth factors,
實驗概要Native chromatin immunoprecipitation to query specific chromatin states of individual genes. 主要試劑10 x TBS 0.1 M Tris-HCl (pH 7.5) 1.5 M NaCl
第一版(2010年9月)前言 醫學論文設計水平的新穎性和創新性,是決定撰寫的醫學論文能否被SCI醫學雜志編委和主編認同的第一門道;醫學論文撰寫水平的邏輯性和規范性,則是決定撰寫的醫學論文能否被SCI醫學雜志接受的最終門道。發表SCI期刊醫學論文的語言是英語,對于母語是非英語的中國臨床醫生
簡明SCI期刊醫學論文撰寫手冊 第一版(2010年9月)前言 醫學論文設計水平的新穎性和創新性,是決定撰寫的醫學論文能否被SCI醫學雜志編委和主編認同的第一門道;醫學論文撰寫水平的邏輯性和規范性,則是決定撰寫的醫學論文能否被SCI醫學雜志接受的最終門道。發表SCI期刊醫學論文的語言
第一版(2010年9月)前言 醫學論文設計水平的新穎性和創新性,是決定撰寫的醫學論文能否被SCI醫學雜志編委和主編認同的第一門道;醫學論文撰寫水平的邏輯性和規范性,則是決定撰寫的醫學論文能否被SCI醫學雜志接受的最終門道。發表SCI期刊醫學論文的語言是英語,對于母語是非英語的中國臨床醫生
實驗概要The method is a native chromatin immunoprecipitation protocol.主要試劑1. 10 x TBS 0.1 M Tris-HCl (pH 7.5) 1.5 M NaCl 30 mM CaCl2 20 mM MgCl2 50 mM Na
1. The preparation of native chromatin from cultured human cells1.1.Cultured cells (e.g. HL-60 or lymphoblastoids) are grown to a density of approxima
For routine transmission electron microscopy (TEM), it is generally accepted that specimens should be thin, dry and contain molecules which diffract e
Mitochondrion-Selective Rhodamines and RosaminesRhodamine 123Rhodamine 123 (R302; FluoroPure Grade, R22420; Figure 12.22) is a cell-permeant, cationic
隨著人類基因組計劃(human genome project )在2003年順利完成,基因組測序技術取得了長足的進步,這直接導致了每兆基因組成本的大幅下降以及檢測的基因組數量越來越多。人們對基因組的復雜性深感震驚,這也引導著測序技術的進一步發展。最近的一些突破性技術使得測序技術在更短的時間內可以
應用WGS正在成為NGS中最廣泛的應用。通過該技術并且結合生物學應用,研究人員可以獲得基因組信息中最值得注意的信息73。舉例來說,2012年,Ellis等報道了基因與乳腺癌患者芳香酶抑制劑(aromatase inhibitor)治療法之間的關聯。他們指出突變,后果與診斷之間的關聯,同樣還有
51. Krupenina NA, Bulychev AA. Action potential-induced changes in photosynthetic pattern of a plant cell
電磁驅動大尺寸MEMS掃描鏡的研究何嘉輝1,2, 周鵬2, 余暉俊2, 沈文江2, 司金海1 摘要:基于微機電系統工藝,設計并制作了一種電磁驅動大尺寸的二維掃描振鏡.分析了兩種不同的電磁驅動方式產生的力的大小,選擇
Sonic Hedgehog (Shh) is a secreted protein identified genetically as an important developmental factor. Shh provides a morphogenic signal in the devel
This diagram illustrates the opposing effects that Proepithelin (PEPI) and the elastase digest fragments, the epithelins (EPIa-g), have on epithelial
9. Spontaneous germination. Some genotypes result in spontaneous ascospore germination (for example, the unpigmented ascospores of per-1 Type I). A si
Seeding After cells are thawed:NOTE: Do not dispense the entire contents of the cryovial into one T-25 flask!!Remove the cap, being car
Figure 5: Regulation of CDH5 by TFZFs in several human cancer cell lines.Blue, cells infected with a pMX construct containing the DNA bindin
My Experience Purifying RNA from E. coliRegarding RNA extraction, there is a horrible tendency of people to use kits for RNA extraction with bacteria.