MethodsfortheDetectionofDAminoAcidOxidase1
AbstractFour methods (an enzyme activity assay, western blotting, RT-PCR, and northern hybridization) to detect the enzyme D-amino-acid oxidase are described.IntroductionD-Amino-acid oxidase catalyzes oxidative deamination of D-amino acids (stereoisomers of naturally occurring L-amino acids) to the corresponding 2-oxo acids, producing ammonia and hydrogen peroxide in the course of the reaction (1). This enzyme is pre......閱讀全文
Methods－for－the－Detection－of－DAminoAcid－Oxidase2
Results and Discussion Navigation Abstract Introduction Materials and Methods Results and Discussion References D-Amino-acid oxi
Methods－for－the－Detection－of－DAminoAcid－Oxidase1
AbstractFour methods (an enzyme activity assay, western blotting, RT-PCR, and northern hybridization) to detect the enzyme D-amino-acid oxidase are de
Coenzyme－A－Detection
實驗概要The experiment provided ?an ?easy, convenient assay to measure the CoA level in a variety of ?biological samples. In the assay, free CoA is specif
Detection－and－Measurement－of－Radioactivity
Radioactive Decay Isotopes of a given element have nuclei with the same number of protons but different numbers of neutrons. Some isotopes are st
Detection－of－Glycoproteins－on－Blot
Detection of Glycoproteins on BlotSource:?Contributed by Sharad Purohit, Paller's LabReagentsSodium acetate Buffer (200mM, pH 5.5): Prepare? a 200
Detection－of－Mycoplasma－by－Culture
AimDetection of mycoplasma by culture is the reference method of detection and has a theoretical level of detection of 1 colony-forming unit (cfu). Ho
Detection－and－Measurement－of－Radioactivity
Liquid scintillation countingThe amount of kinetic energy in a beta particle differs from one decay to the next. However, each radioisotope has a typi
Detection－by－TUNEL－labeling
In Situ Cell Death (Apoptosis) Detection by TUNEL labelingby Boehringer Mannheim (Catalog No. 1684809), modified by Josiah N. Wilcox,José C. Rodriguez
FACS－Procedures－for－Apoptosis－Detection
Materials:Hoechst?33258?(Sigma B-2883).stock: 10 mg/ml in dH20 (40)working dilution: 500μg/ml (50μl stock + 950μl PBS).7-Amino-actinomycin (Sigma A-94
Bespoke－Metal－Detection－Conveyor－Systems
Many metal detection applications do not fit into the scope of ? standard conveyor systems. For this reason, METTLER TOLEDO SAFELINE are able to
Protein－detection－onto－PVDF－membranes
2-D PAGE and electroblotting onto PVDF membranes have become widely used techniques for the characterization of proteins. Recent improvements have al
Detection－of－Intracellular－Antigens－by－Flow－Cytometry
實驗概要Fix and Perm ?reagents are designed for use with all commercially available flow ?cytometers. Alignment and compensation should be performed accor
Cell－death－detection－in－Xenopus－embryos－by－ELISA
Sample preparation Wash embryos 1 x in 25% MMR Remove excess buffer Add 10 volumes "incubation buffer", i.e. 50 μl for 5 embryos Lyse the embryos
Detection－of－apoptotic－process－in－situ－using－immunocytochemical
1. INTRODUCTION??Apoptosis was observed from invertebrates to lower and higher verterbrates, and intervenes both in physiological and in pathological
Detection－of－BrdU－Incorporation－in－DNA－Synthesizing－Cells
Detection of BrdU Incorporation in DNA Synthesizing Cells?NOTE:?Bromodeoxyuridine is a known carcinogen. Propidium iodine (PI) is known to be toxic an
PCR－Primers－For－Gene－Expression－Detection－or－Quantification
Why PrimerBank?PrimerBank is a public resource for PCR primers. These primers are designed for gene expression detection or quantification (real-time
Comparison－of－lysis－methods
Comparison of lysis methods Sonication most efficient method of cell fractionation problem: heat build up which can denature proteins (proport
Staining－Methods－for－cell
death Z. Xia 10/2/95 The simplest way: trypan blue.? Dead cells stain blue Non-fixed cells: FDA(fluorescein diacetate)-green, alive cells;?
Guide－to－Cell－Proliferation－and－Apoptosis－Methods
Chapter 1: Cell Death - Apoptosis and Necrosis 1.1 Introduction 2 1.1.1 Terminology of cell death 2 1.1.2 Differences between necros
In－Situ－Cell－Death－(Apoptosis)－Detection－by－TUNEL－labeling
Protocol for Frozen Sections:Warm 150ml 4% Paraformaldehyde/1x PBS to RT. Fix slides in it, 20 min., RT.1x PBS rinse, 2 times.1x PBS, 30 min., RT. Beg
Detection－Of－Cell－Viability－And/Or－Apoptosis－By－Flow－Cytometry－(FACS)
Viable?cells are cells that when allowed to continue beyond the timepoint of examination will stay alive. Besides live and healthy cells, cells in ear
Detection－of－MicroRNA－Heterogeneity－in－Single－Cells－Using－an－Automated
Introduction ?MicroRNA ?(miRNAs) are short (18–24 nucleotides), non-coding RNAs that regulate ?gene expression by both disrupting messenger RNA (mRNA
Staining－Procedure－for－Flow－Cytometric－Detection－of－Human－Cyclins
Staining Procedure for Flow Cytometric Detection of Human CyclinsThis is a standard protocol used at Pharmingen for Quality Control testing of the ant
Determination－and－Detection－of－Reactive－Oxygen－Species－(ROS),－Lipid－...
Reactive oxygen species or intermediates are formed by the incomplete reduction of oxygen. Organisms living in aerobic environment generate variou
General－Laboratory－Methods－for－Tetrapyrroles
There are thousands of porphyrins and metalloporphyrins, and hundreds of new derivatives appear each year. This variety arises because the cyclic
Plant－Genome－Annotation－Methods
Annotation of plant genomic sequences can be separated into structural and functional annotation. Structural annotation is the foundation of all
Analysis－and－Reconstitution－of－Phycobiliproteins:－Methods－for－the－...
Analysis and Reconstitution of Phycobiliproteins: Methods for the Characterization of Bilin Attachment Reactions Phycobiliproteins are a homologous
Staining－Methods－for－cell－death
The simplest way: trypan blue.? Dead cells stain blue Non-fixed cells: FDA(fluorescein diacetate)-green, alive cells;? P.I. (propidium iodide)-r
Basic－Methods－of－Culturing－Drosophila
實驗概要Basic Methods of Culturing Drosophila實驗步驟Stockkeeping1. Mechanics? ? ? ? Most stocks can be successfully cultured by periodic mass transfer of a
Methods－for－Xylem－Sap－Collection
Xylem and phloem are essential for the exchange of solutes and signals among organs of land plants. The synergy of both enables the transport and